0000000000016542

AUTHOR

Domenico Galati

showing 4 related works from this author

Analysis of Mycobacterium tuberculosis-Specific CD8 T-Cells in Patients with Active Tuberculosis and in Individuals with Latent Infection

2009

CD8 T-cells contribute to control of Mycobacterium tuberculosis infection, but little is known about the quality of the CD8 T-cell response in subjects with latent infection and in patients with active tuberculosis disease. CD8 T-cells recognizing epitopes from 6 different proteins of Mycobacterium tuberculosis were detected by tetramer staining. Intracellular cytokines staining for specific production of IFN-gamma and IL-2 was performed, complemented by phenotyping of memory markers on antigen-specific CD8 T-cells. The ex-vivo frequencies of tetramer-specific CD8 T-cells in tuberculous patients before therapy were lower than in subjects with latent infection, but increased at four months a…

MaleEpitopes T-Lymphocytelcsh:MedicineCD8-Positive T-LymphocytesEpitopeDiagnostic RadiologyInfectious Diseases/Bacterial InfectionsSpectrum Analysis TechniquesCellular typesCytotoxic T celllcsh:ScienceImage Cytometryeducation.field_of_studyMultidisciplinarybiologyRadiology and ImagingImmune cellsInfection ImagingMiddle AgedFlow CytometryActinobacteriaPhenotypeSpectrophotometryCytokinesWhite blood cellsFemaleCytophotometryResearch Articlemedicine.drugInterleukin 2Cell biologyBlood cellsTuberculosisImaging TechniquesImmunologyPopulationT cellsCytotoxic T cellsResearch and Analysis MethodsMycobacterium tuberculosisDiagnostic MedicineImmunology/Immunity to InfectionsHLA-A2 AntigenmedicineHumansTuberculosiseducationMedicine and health sciencesHLA-A AntigensBacteriaFluorimetrylcsh:ROrganismsBiology and Life SciencesMycobacterium tuberculosisbiology.organism_classificationmedicine.diseaseVirologyRetractionAnimal cellsImmunology/Immune ResponseImmunologyMycobacterium tuberculosis CD8 T cells Tuberculosis Latent Infectionlcsh:QCD8MycobacteriumPLoS ONE
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Multifunctional CD4(+) T cells correlate with active Mycobacterium tuberculosis infection.

2010

Th1 CD4(+) T cells and their derived cytokines are crucial for protection against Mycobacterium tuberculosis. Using multiparametic flow cytometry, we have evaluated the distribution of seven distinct functional states (IFN-gamma/IL-2/TNF-alpha triple expressors, IFN-gamma/IL-2, IFN-gamma/TNF-alpha or TNF-alpha/IL-2 double expressors or IFN-gamma, IL-2 or TNF-alpha single expressors) of CD4(+) T cells in individuals with latent M. tuberculosis infection (LTBI) and active tuberculosis (TB). We found that triple expressors, while detectable in 85-90%TB patients, were only present in 10-15% of LTBI subjects. On the contrary, LTBI subjects had significantly higher (12- to 15-fold) proportions of…

Interleukin 2AdultCD4-Positive T-LymphocytesMaleTuberculosisSettore MED/17 - Malattie InfettiveImmunologyCell SeparationBiologyLymphocyte ActivationFlow cytometryMycobacterium tuberculosis03 medical and health sciences0302 clinical medicineImmune systemBacterial ProteinsCD4(+) T cells Cytokines Mycobacterium tuberculosis infection Tuberculosis disease interferon-gamma immunological memory disease responses protection cytokine immunity bcg vaccination virusmedicineImmunology and AllergyDistribution (pharmacology)HumansCytokineTuberculosis Pulmonary030304 developmental biologyTuberculosis disease.Settore MED/04 - Patologia Generale0303 health sciencesAntigens Bacterialmedicine.diagnostic_testMycobacterium tuberculosis infectionMycobacterium tuberculosisMiddle Agedbiology.organism_classificationmedicine.diseaseFlow CytometryPhenotypeVirologyCD4+ T cellsBacterial Load3. Good healthImmunologyAcute DiseaseChronic DiseaseCytokinesTumor necrosis factor alphaAcyltransferases030215 immunologymedicine.drugEuropean journal of immunology
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Sulodexide versus calcium heparin in the medium-term treatment of deep vein thrombosis of the lower limbs.

1997

Thirty adult patients with distal, monolateral deep vein thrombosis of the lower limbs were randomly treated for sixty days either with subcutaneous Ca-Heparin or with Sulodexide, administered IM for ten days and orally for fifty days. The thrombus accretion above the knee, the venous pressures of the affected leg, the clinical sympto matology, and some laboratory coagulative tests were monitored throughout the admin istration period. Local tolerability of the two treatments was also evaluated.The two applied treatments evidenced a net antithrombotic activity, preventing thrombus accretion above the knee, improving with the same efficacy the venous pressures in the affected legs, and simila…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina Internamedicine.drug_classDeep vein030204 cardiovascular system & hematology03 medical and health sciences0302 clinical medicineFibrinolytic AgentsHumansMedicine030212 general & internal medicineThrombusAgedGlycosaminoglycansmedicine.diagnostic_testHeparinbusiness.industryAnticoagulantHeparinMiddle AgedThrombophlebitismedicine.diseaseThrombosisSulodexideSurgerymedicine.anatomical_structureTolerabilityAnesthesiaSettore BIO/14 - FarmacologiaFemaleAdult Aged Female Fibrinolytic Agents/administration & dosage Fibrinolytic Agents/therapeutic use* Glycosaminoglycans/administration & dosage Glycosaminoglycans/therapeutic use* Heparin/therapeutic use* Humans Male Middle Aged Thrombophlebitis/drug therapy*Cardiology and Cardiovascular Medicinebusinessmedicine.drugPartial thromboplastin time
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Evaluation of regional haemodynamics and alterations of vascular wall of the lower limbs in hypertensive subjects

1995

This study was designed to analyse the relationship between arterial hypertension and changes in arterial blood flow and vascular wall damage of the lower limbs in hypertensive patients with various degrees of hypertension. Six hundred and fifty-four hypertensive patients (421 males and 233 females) aged 35 to 70 years and 88 healthy subjects (63 males and 25 females) aged 39 to 60 years were studied. Strain-gauge plethysmography of the lower limbs was used to calculate arterial calf blood flow (RF), arterial calf blood flow after post-ischaemic hyperaemia (PF), basal and minimal vascular resistances (BVR and MVR), time to reach peak flow (tPF), time until 50% reduction of peak flow (tT1/2)…

AdultMaleDuplex ultrasonographymedicine.medical_specialtySettore MED/09 - Medicina InternaHemodynamicsEssential Hypertension Arterial blood flow of the lower limbs medial-intima thickeningHyperaemiaInternal medicinemedicine.arterymedicinePlethysmographHumansKneeAgedUltrasonographyLegbusiness.industryHemodynamicsBlood flowArteriesMiddle AgedSettore MED/11 - Malattie Dell'Apparato CardiovascolarePopliteal arterySurgeryMean blood pressuremedicine.anatomical_structureRegional Blood FlowHypertensionCardiologyFemaleVascular Resistancemedicine.symptomCardiology and Cardiovascular MedicinebusinessTunica IntimaTunica MediaArtery
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