0000000000020572
AUTHOR
Monika Meyer
showing 3 related works from this author
Incorporation of membrane proteins into lipid surface monolayers: Characterization by fluorescence and electron microscopies
2007
The preparation of oriented protein samples is an attractive goal, e.g., to gain more detailed information from spectroscopic experiments. Our approach towards this aim was to prepare monolayers of phospholipids at the air-water interface and to incorporate the proteins into these ordered structures. Subsequently, we used the Langmuir-Boldgett (LB) transfer technique to obtain samples of oriented proteins on solid supports. — Incorporation was achieved by spreading the proteins from a detergent solution onto a prespread lipid monolayer on the water surface. We characterized successful incorporation by in situ fluoresence microscopy and by electron microscopy, and investigated the topology o…
Pigment-pigment interactions and secondary structure of reconstituted algal chlorophyll a/b-binding light-harvesting complexes of Chlorella fusca wit…
1995
Earlier we have shown by in vitro reconstitution experiments that the pigment composition of the chlorophyll alb-binding light-harvesting complex of the green alga Chlorella fusca could be altered in a relatively broad range (Meyer and Wilhelm 1993). In this study we used these reconstituted complexes of different pigment loading to analyze the excitonic interactions between the pigment molecules and the secondary structure by means of circular dichroism spectra in the visible and the far UV spectral regions, respectively. We found that, in contrast to the expectations, the pigment composition and pigment content hardly affected the circular dichroism spectra in the visible spectral region.…
Reconstitution of Light-Harvesting Complexes from Chlorella fusca (Chlorophyceae) and Mantoniella squamata (Prasinophyceae)
1993
Abstract Reconstitution experiments of light-harvesting complexes were performed with the green alga Chlorella fusca and the chlorophyll c-containing prasinophyte Mantoniella squamata using a modified method according to Plumley and Schmidt [Proc. N atl. Acad. Sei. U .S.A . 84, 146 -150 (1987)]. Changing the pigment supply quantitatively or qualitatively in the reconstitution mixture homologous and heterologous reconstitutes were obtained. In contrast to higher plants, light-harvesting polypeptides from green algae are able to bind the chlorophylls as well as the xanthophylls in different stoichiometries. Heterologous reconstitutes of M . squamata polypeptides give further evidence for a r…