0000000000022024

AUTHOR

Chiara Totta

showing 2 related works from this author

Temporal frames of 45S rDNA site-number variation in diploid plant lineages: lessons from the rock rose genus Cistus (Cistaceae)

2016

The perception that the turnover of 45S rDNA site number in plants is highly dynamic pervades the literature on rDNA evolution. However, most reported evidences come from the study of polyploid systems and from crop species subjected to intense agronomic selection. In sharp contrast with polyploids, the evolutionary patterns of rDNA loci number in predominantly diploid lineages have received less attention. Most studies on rDNA loci changes lack explicit temporal frames, and hence their dynamics could not be assessed. Here, we assess the temporal patterns of rDNA site evolution in Cistus, an entirely diploid lineage. We assessed the number and chromosomal position of 45S rDNA loci in Cistus…

0301 basic medicinebiologyCistusTuberariaCistaceaeCistaceaebiology.organism_classification45S rDNATuberaria03 medical and health sciencesFumana030104 developmental biologyGenusPhylogeneticsCistusBotanyfluorescent in situ hybridizationFumanaPloidydiploid lineageRibosomal DNAEcology Evolution Behavior and Systematics
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Nuclear rDNA instability in in vitro-generated plants is amplified after sexual reproduction with conspecific wild individuals

2016

Using micropropagation through tissue culture has become the most used approach worldwide for mass production for the conservation of endangered species. However, the screening of somaclonal variations generated using in vitro culture is usually restricted to the first generation of micropropagated plants, when they have not yet been released in the field. Accordingly, the fate of genetically modified regenerants after sexual reproduction is usually not assessed and changes in the genetic structures of species are unknown. In this work, we assess the cytogenetic stability of two rDNA gene families in the offspring of experimental crosses between accessions generated after in vitro culture a…

0106 biological sciencesPlant ScienceCistaceaeBiologybiology.organism_classification010603 evolutionary biology01 natural sciencesGenetically modified organismSexual reproductionTissue cultureMicropropagationCistusGenetic structureBotanyGene familyEcology Evolution Behavior and Systematics010606 plant biology & botany
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