0000000000023534

AUTHOR

Leopoldo Palma

showing 2 related works from this author

Vip3C, a novel class of vegetative insectidal proteins from Bacillus thuringiensis

2012

Three vip3 genes were identified in two Bacillus thuringiensis Spanish collections. Sequence analysis revealed a novel Vip3 protein class (Vip3C). Preliminary bioassays of larvae from 10 different lepidopteran species indicated that Vip3Ca3 caused more than 70% mortality in four species after 10 days at 4 οg/cm 2. © 2012, American Society for Microbiology.

DNA BacterialBioquímicaSequence analysisMolecular Sequence DataBiotecnologia agrícolaBacillus thuringiensisBiologyApplied Microbiology and BiotechnologyLepidoptera genitaliaBacterial proteinPlagues ControlBacterial ProteinsPhylogeneticsBacillus thuringiensisBotanyPlaguicidesInvertebrate MicrobiologyAnimalsBioassayGenePhylogenyLarvaSequence Homology Amino AcidEcologyfungiSequence Analysis DNAbiology.organism_classificationSurvival AnalysisVip3 genesLepidopteraSpainLarvaProteïnesFood ScienceBiotechnology
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Insecticidal spectrum and mode of action of the Bacillus thuringiensis Vip3Ca insecticidal protein.

2016

The Vip3Ca protein, discovered in a screening of Spanish collections of Bacillus thuringiensis, was known to be toxic to Chrysodeixis chalcites, Mamestra brassicae and Trichoplusia ni. In the present study, its activity has been tested with additional insect species and we found that Cydia pomonella is moderately susceptible to this protein. Vip3Ca (of approximately 90 kDa) was processed to an approximately 70 kDa protein when incubated with midgut juice in all tested species. The kinetics of proteolysis correlated with the susceptibility of the insect species to Vip3Ca. The activation was faster to slower in the following order: M. brassicae (susceptible), Spodoptera littoralis (moderately…

0301 basic medicineInsecticides030106 microbiologyInsect pest controlAgrotis ipsilonVegetative insecticidal proteinsMothsmedicine.disease_causeMicrobiologyCiencias BiológicasInsecticide Resistance03 medical and health sciencesBiología Celular MicrobiologíaBacterial ProteinsBacillus thuringiensisBotanyTrichoplusiamedicineAnimalsSpodoptera littoralisPest Control BiologicalEcology Evolution Behavior and SystematicsHistological localizationbiologyToxinfungiVEGETATIVE INSECTICIDAL PROTEINSMidgutBioinsecticidesApical membranebiology.organism_classificationCROP PROTECTIONChrysodeixis chalcitesBIOINSECTICIDES030104 developmental biologyCrop protectionINSECT PEST CONTROLHISTOLOGICAL LOCALIZATIONCIENCIAS NATURALES Y EXACTASJournal of invertebrate pathology
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