0000000000023739
AUTHOR
Zuo-fu Wei
Activity of the antiestrogenic cajanin stilbene acid towards breast cancer
Antiestrogenic therapy is a mainstay for estrogen receptor (ERα)-positive breast cancer. Due to the development of resistance to established antihormones such as tamoxifen, novel compounds are required. The low abundant cajanin stilbene acid (CSA) recently isolated by us from Pigeon Pea (Cajanus cajan) has structural similarities with estrogen. We analyzed the cytotoxic and anticancer activity of CSA in ERα-positive and -negative human breast cancer cells in vitro, in vivo and in silico. CSA exerts anticancer and antiestrogenic activities towards ERα-positive breast cancer, and it showed cytotoxicity towards tamoxifen-resistant MCF-7 cells, implying that CSA may be active against tamoxifen-…
Resin adsorption as a means to enrich rare stilbenes and coumarin from pigeon pea leaves extracts
Abstract In this study, resin adsorption as a means to enrich rare stilbenes cajaninstilbene acid (CSA) and longistyline C (LLC), and coumarin cajanuslactone (CL) from pigeon pea leaves was investigated. Among widely used twenty macroporous adsorption resins, NKA-9 resin presented higher adsorption capacity and desorption ratio in static tests. The adsorption kinetic model fitted better depended on types of compounds and pH values. The experimental adsorption data were better fitted to Langmuir isotherm (R2 0.921–0.991) than Freundlich isotherm (R2 0.753–0.988). In order to optimize the operating parameters for separating CSA, LLC and CL, dynamic adsorption and desorption tests were carried…
Negative-pressure cavitation extraction of cajaninstilbene acid and pinostrobin from pigeon pea [Cajanus cajan (L.) Millsp.] leaves and evaluation of antioxidant activity
Abstract In this study, an efficient negative-pressure cavitation extraction (NPCE) technique was developed to extract cajaninstilbene acid (CSA) and pinostrobin (PI) from pigeon pea [Cajanus cajan (L.) Millsp.] leaves. The operating parameters were optimised using single-factor experiment and central composite design combined with response surface methodology. Well-fitting models for the extraction of CSA and PI were successfully established. From the economic perspective, the selected operating parameters were: extraction pressure −0.075 MPa, extraction temperature 45 °C, material size 40 mesh, ethanol concentration 80%, extraction time 30 min, liquid/solid ratio 30:1 and 4 extraction cyc…
Variation in contents of main active components and antioxidant activity in leaves of different pigeon pea cultivars during growth.
Pigeon pea is an important and multiuse grain legume crop, and its leaves are a very valuable natural resource. To obtain a high-quality biological resource, it is necessary to choose the excellent cultivar and determine the appropriate harvest time. In this study, the variation in contents of main active components and antioxidant activity in leaves of six pigeon pea cultivars during growth were investigated. The level of each individual active component significantly varied during growth, but with a different pattern, and this variation was different among cultivars. Flavonoid glycosides orientin, vitexin, and apigenin-6,8-di-C-α-L-arabinopyranoside showed two peak values at mid-late and …
UV-Induced Changes of Active Components and Antioxidant Activity in Postharvest Pigeon Pea [Cajanus cajan (L.) Millsp.] Leaves
In this study, the effect of UV irradiation (UV-A, UV-B, and UV-C) on phytochemicals, total phenolics, and antioxidant activity of postharvest pigeon pea leaves was evaluated. The response of pigeon pea leaves to UV irradiation was phytochemical specific. UV-B and UV-C induced higher levels of phytochemicals, total phenolics, and antioxidant activity in pigeon pea leaves compared with UV-A. Furthermore, UV-B irradiation proved to possess a long-lasting effect on the levels of phenolics and antioxidant activity. After adapting for 48 h at 4 °C following 4 h UV-B irradiation, total phenolics and antioxidant activity were approximately 1.5-fold and 2.2-fold increased from 39.4 mg GAE/g DM and …
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajan in breast cancer cells
Abstract Background: The low abundant cajanin stilbene acid (CSA) from Pigeon Pea ( Cajanus cajan ) has been shown to kill estrogen receptor α positive cancer cells in vitro and in vivo . Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet. Material and methods: We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis. Results: CSA induced G2/M arrest and apoptosis …