0000000000025442

AUTHOR

Domenico Taverna

showing 3 related works from this author

miR-21 antagonism abrogates Th17 tumor promoting functions in multiple myeloma

2020

Multiple myeloma (MM) is tightly dependent on inflammatory bone marrow microenvironment. IL-17 producing CD4+ T cells (Th17) sustain MM cells growth and osteoclasts-dependent bone damage. In turn, Th17 differentiation relies on inflammatory stimuli. Here, we investigated the role of miR-21 in Th17-mediated MM tumor growth and bone disease. We found that early inhibition of miR-21 in naive T cells (miR-21i-T cells) impaired Th17 differentiation in vitro and abrogated Th17-mediated MM cell proliferation and osteoclasts activity. We validated these findings in NOD/SCID-g-NULL mice, intratibially injected with miR-21i-T cells and MM cells. A Pairwise RNAseq and proteome/phosphoproteome analysis…

0301 basic medicineMaleCancer ResearchBone diseaseApoptosisBone NeoplasmsNodMice SCIDBone NeoplasmT-Lymphocytes RegulatoryTh17 Cell03 medical and health sciencesMice0302 clinical medicineDownregulation and upregulationgammopathiesMice Inbred NODmedicineTumor Cells CulturedTumor MicroenvironmentBiomarkers TumorAnimalsHumansMultiple myelomaCell ProliferationChemistryCell growthAnimalApoptosiHematologymedicine.diseasePrognosisXenograft Model Antitumor AssaysIn vitroGene Expression Regulation NeoplasticMicroRNAs030104 developmental biologymedicine.anatomical_structureOncology030220 oncology & carcinogenesisCase-Control StudiesCancer researchTh17 CellsBone marrowAntagonismCase-Control StudieMultiple Myeloma
researchProduct

Rapid assay of resveratrol in red wine by paper spray tandem mass spectrometry and isotope dilution.

2017

A rapid analytical approach for the assay of resveratrol in red wines, based on Paper Spray Mass Spectrometry (PS-MS) and Multiple Reaction Monitoring (MRM) is described. The assay involves the use of the stable isotope dilution method. The analytical parameters calculated analyzing fortified samples confirm the reliability of the proposed approach, with accuracy values about 100%, and LOD and LOQ values calculated at 0.5 and 0.8 μg/mL, respectively. Furthermore, both the recovery, which was quantitative for the analyte, and the reproducibility (RSD%), checked on different days on the same wine, always below 7%, highlighted the consistency of the methodology.

AnalyteWineFood chemistryIsotope dilution010402 general chemistryMass spectrometryTandem mass spectrometry01 natural sciencesQuantitative assayAnalytical ChemistryAmbient mass spectrometryIsotopesTandem Mass SpectrometryStilbenesWineReproducibilityChromatographyPaper sprayIsotopeChemistryMedicine (all)010401 analytical chemistrySelected reaction monitoringGeneral MedicineIsotope dilution0104 chemical sciencesResveratrolStilbeneFood ScienceFood chemistry
researchProduct

Determination of ketosteroid hormones in meat by liquid chromatography tandem mass spectrometry and derivatization chemistry.

2015

A method for the determination and quantification of ketosteroid hormones in meat by mass spectrometry, based on the derivatization of the carbonyl moiety of steroids by O-methylhydroxylamine, is presented. The quantitative assay is performed by means of multiple-reaction-monitoring (MRM) scan mode and using the corresponding labelled species, obtained by reaction with d 3-methoxylamine, as internal standard. The accuracy of the method was established by evaluating artificially spiked samples, obtaining values in the range 90-110%. Recovery tests were performed on blank matrix samples spiked with non-natural steroids including trenbolone and melengestrol acetate. The latter experiment revea…

Multiple reaction monitoringChromatographyMeatMass spectrometrySelected reaction monitoringKetosteroidMass spectrometrySolid-phase microextractionKetosteroidsBiochemistryAnalytical ChemistryMatrix (chemical analysis)chemistry.chemical_compoundTrenbolonechemistryLiquid chromatography–mass spectrometryTandem Mass SpectrometryKetosteroidMethoxylamineUHPLCmedicineDerivatizationSolid Phase Microextractionmedicine.drugAnalytical and bioanalytical chemistry
researchProduct