0000000000030816
AUTHOR
Anna Taravella
Comparative Cytogenetic Analysis of Three Stylommatophoran Slugs (Mollusca, Pulmonata)
system is still controversial because phylogeny and systematic relationships at the family level are poorly understood. Besides morphological studies, ribosomal RNA genes and the H3/H4 histone gene cluster (Ambruster et al., 2005; Wade et al., 2006) have also been used to resolve the relationships within this order. Recently, by comparison of primary sequence of mitochondrial and nuclear genes, Grande et al. (2004) resolved the Stylommatophora as the early split monophyletic sister group of all the other gastropod taxa. Available data on the cytogenetics of Stylommatophora are extremely poor (reviewed by Patterson, 1969, and Thiriot-Quievreux, 2003) and mostly concern the haploid (n) and/ o…
Optimized RNA extraction and northern hybridization in streptomycetes.
Abstract Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity and recovery and unbiased transfer of the entire transcripts population. Many protocols suffer from severe limitations including only partial protection of the RNA integrity and/or loss of small sized molecules. Moreover, some of them do not allow an efficient and even transfer in the entire sizes range. These difficulties become more prominent in streptomycetes, where an initial quick lysis step is difficult to obtain. We present here an optimized northern hybridization protoc…