0000000000038604

AUTHOR

C. Nicotra

showing 3 related works from this author

Binding of 11-cis retinaldehyde to the partially purified cellular retinaldehyde binding protein from bovine retinal pigment epithelium.

1987

11-cis retinaldehyde binding analysis was performed on a bovine retinal pigment epithelium preparation of cellular retinaldehyde binding protein (CRALBP), whose purity degree was estimated as 75%. Equilibrium binding studies were carried out measuring the replacement of tritium-labeled with unlabeled 11-cis retinaldehyde at 25 degrees C. Analysis of the experimental data both by a direct curve-fitting procedure utilizing a non linear least square regression analysis and by a conventional Scatchard plot revealed a single non-interacting binding site with an apparent equilibrium constant of 0.9 X 10(-7) M. A binding stoichiometry of approximately 1 mol of 11-cis retinaldehyde/mol of binding p…

Apparent Equilibrium ConstantBiologyBinding CompetitiveCellular and Molecular Neurosciencechemistry.chemical_compoundRetinoidsmedicineAnimalsBinding sitePigment Epithelium of EyeMolecular BiologyPharmacologyRetinaRetinal pigment epitheliumBinding proteinRetinalCell BiologyKineticsmedicine.anatomical_structureBiochemistrychemistryCELLULAR RETINALDEHYDE-BINDING PROTEINRetinaldehydeRetinaldehydeMolecular MedicineCattleCarrier ProteinsExperientia
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Retinol and retinyl esters in pigment epithelium of rats with inherited retinal degeneration

1976

A comparative study of the retinol and retinyl ester concentrations was performed in the retinal pigment epithelium of the normal and affected rats. Our findings indicate that in dystrophic rat retinol content increases, whereas the amount of retinyl esters is always lower than normal. An hypothesis can be made on the deficiency of enzymic activities which regulate retinol retinol and retinyl esters levels in the pigment epithelium.

Retinal degenerationgenetic structuresRetinyl estersCellular and Molecular NeurosciencePigmentchemistry.chemical_compoundmedicineAnimalsPigment Epithelium of EyeVitamin AMolecular BiologyPharmacologyRetinal pigment epitheliumChemistryRetinal DegenerationAge FactorsRetinolCell Biologymedicine.diseaseEpitheliumRatsmedicine.anatomical_structureBiochemistryvisual_artvisual_art.visual_art_mediumMolecular MedicineExperientia
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Polyamines and ripening of photoreceptor outer segments in chicken embryos.

1995

Abstract Polyamines and their related monoacetyl derivatives were studied in rod outer segment (ROS) and cone outer segment (COS) of photoreceptor cells from chick embryo retina during eye development (7th–18th days). Putrescine was found to be necessary, in the second phase of retinogenesis, to sustain both ROS and COS differentiation and, after acetylation, γ-aminobutyric acid synthesis. On the other hand, spermidine and even more spermine intervene in the third phase of development when photoreceptors mature. Moreover, the presence of N1-acetylspermidine already at the 7th day indicates that in the outer segment of photoreceptor cells too, as in the whole retina, putrescine synthesis com…

genetic structuresSpermineChick EmbryoBiologyOrnithine DecarboxylaseRetinaOrnithine decarboxylasechemistry.chemical_compoundDevelopmental NeuroscienceAcetyltransferasesCadaverinemedicinePutrescineAnimalsPhotoreceptor Cellsgamma-Aminobutyric AcidRetinaBiogenic PolyaminesCell DifferentiationRod Cell Outer SegmentSpermidinemedicine.anatomical_structurechemistryBiochemistrySpermine synthasebiology.proteinPutrescineSperminesense organsSpermidine synthasePolyamine oxidaseDevelopmental BiologyInternational journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
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