0000000000040076
AUTHOR
Lada Bumbure
Combined Effects of 50 Hz Electromagnetic Field and SiO2 Nanoparticles on Oxidative Stress in Plant’s Gametic Cells / Zemfrekvences (50 Hz) Elekromagnētiskā Lauka Un SiO2 Nanodaļiņu Kombinētā Ietekme Uz Augu Gametisko Šūnu Oksidatīvo Stresu
Abstract The paper presents the results of combined effects of an extremely low frequency electromagnetic field (50 Hz) (ELF EMF) and SiO2 nanoparticles on fluorescence of plant gametic cells (immature microspores). The data were recorded by a BD FACSJazz® cell sorter after cell irradiation by blue laser (488 nm). A significant difference of fluorescence was observed between gametic cells after 1 hour incubation in suspension of SiO2 nanoparticles and the control gametic cells. It was observed that fluorescence intensity of gametic cell was higher with ELF EMF treatment in comparison to control cells, but it was statistically significant only for cells treated with electromagnetic radiation…
Amber Particles as Living Plant Cell Markers in Flow Cytometry / Dzintara Daļiņas Kā Dzīvu Augu Šūnu Marķieri Plūsmas Citometrijā
Abstract The unique biological properties of amber are well known. Amber particles that penetrate into the cellular matrix can potentially be used as markers of plant cell biological activity by identification of living cells in flow cytometry. However, there have been no studies on effect of amber particles on plant cells. The aim of this study was to determine effect of amber nano- and micro- (5 nm-3 μm) particles on somatic and gametic cells and to assess the possibility to use amber particles as alive plant cells markers. To reach the aim, fluorescence of cells was determined in the presence of amber particles, and amber components - spirit of amber and sodium succinate dibasic hexahydr…
Influence of SiO2 nanoparticles on relative fluorescence of plant cells
Nanoparticles (nano-scale particles (NSPs)) are defined as particles with dimensions less than 100 nm. SiO<sub>2</sub> nanoparticles are one of the most widely common nanoparticles in the environment, particularly in urban areas. The sources of SiO<sub>2 </sub>nanoparticles are very different, including natural nanoparticles, anthropogenic and engineered nanoparticles. The SiO<sub>2 </sub>nanoparticles could be considered a source of different pollution effects on leaving organisms. Nevertheless, knowledge of the mechanisms, through which the SiO<sub>2 </sub>nanoparticles affect cells, is incomplete. The aim of the research was to elaborate a …