0000000000043348

AUTHOR

Davide Calascibetta

showing 3 related works from this author

Study of β-defensin polymorphisms in Valle del Belice dairy sheep

2009

The aim of this work was to sequence the exons of β-defensin 1 and 2 genes (SBD1 and SBD2) in Valle del Belice dairy sheep in order to identify polymorphisms. The study was conducted on 60 samples from three flocks. Six SNPs were identified: two in SBD1 and four in SBD2. Both genes consist of two exons and one intron. In SBD1 gene, SNPs were found only in the exon 2, whereas in SBD2, SNPs were detected in both exons. In both genes, SNPs were located in the coding regions and in the 3'-UTR. The SNP in SBD2 located at position 1659 determined a change in the protein sequence. Further studies will be necessary to investigate if the amino acid change modifies the biological function of the…

GeneticsUntranslated regionsheep040301 veterinary sciences0402 animal and dairy scienceIntronβ-defensin SNP Sheep.SNPSingle-nucleotide polymorphism04 agricultural and veterinary sciencesBiology040201 dairy & animal scienceMolecular biologyβ-defensinβ-defensin; SNP; sheep0403 veterinary scienceExonSettore AGR/17 - Zootecnica Generale E Miglioramento GeneticoSNPCoding regionAnimal Science and Zoologylcsh:Animal cultureDefensinGenelcsh:SF1-1100
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Sequence characterization of the melanocortin 1 receptor (MC1R) gene in sheep with different coat colour and identification of the putative e allele …

2010

Abstract Sequence of the melanocortin 1 receptor (MC1R) gene (the Extension locus) was obtained from a panel of 73 animals belonging to 9 Italian sheep breeds or populations (Appenninica, Bergamasca, Comisana, Cornigliese-like, Delle Langhe, Massese, Merinizzata Italiana, Sarda and Valle del Belice) with different coat colours. Evaluation of the identified polymorphisms on this phenotype was reported with in silico predictions and comparative approaches within and across breeds and across species. Five novel single nucleotide polymorphisms (SNPs), organized in three haplotypes, were detected. Another haplotype, including the two missense mutations already described for the ED allele, was id…

GeneticsHaplotypeCoat colour; MC1R; Missense mutation; Ovine breedsSingle-nucleotide polymorphismLocus (genetics)BiologyOvine breedsSettore AGR/17 - Zootecnica Generale E Miglioramento GeneticoCOAT COLOURFood AnimalsSHEEPBREEDSMC1REpistasisAnimal Science and ZoologyMissense mutationAlleleGeneAllele frequencyPOLYMORPHISMSMelanocortin 1 receptor
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Polymorphisms of β-defensin genes in Valle del Belice dairy sheep

2011

The aim of this work was to study β-defensin 1 (SBD1) and β-defensin 2 (SBD2) genes in Valle del Belice dairy sheep in order to identify polymorphisms that can be utilized as markers of the analyzed genes, and search for the functional effects and roles of the identified polymorphisms (variation of the amino acid sequence of the protein and stability of mRNA molecule). The study was conducted on 300 randomly selected animals belonging to four flocks. A total of seven SNPs were identified, two in SBD1 and five in SBD2. The two SNPs identified in SBD2 coding region, at position 1659 and position 1667, were non-synonymous, leading to amino acid changes in the protein product. Nevertheless, the…

Untranslated regionbeta-DefensinsGenotypeMolecular Sequence DataSingle-nucleotide polymorphismBiologyPolymorphism Single NucleotideSettore AGR/17 - Zootecnica Generale E Miglioramento GeneticoGene Frequencyb-Defensin SNPs Sheep Valle del BeliceGeneticsAnimalsCoding regionRNA MessengerMolecular BiologyDefensinGenePeptide sequenceSheep Domesticchemistry.chemical_classificationGeneticsBase SequenceComputational BiologyGeneral MedicineMolecular biologyAmino acidDairyingchemistryGenetic markerNucleic Acid ConformationMolecular Biology Reports
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