0000000000049442
AUTHOR
Harri Leppänen
The use of fish bile metabolite analyses as exposure biomarkers to pulp and paper mill effluents
The exposure of caged one-year-old whitefish (Coregonus lavaretus) to wood extractives and chlorophenolics from pulp and paper mill effluents in Lake Saimaa (SE Finland) was investigated. Whitefish were exposed at 17 locations affected by effluents from pulp and paper mills and at 5 reference areas. Resin and fatty acids and chlorophenolics in bile were analysed by GC and GC-MS. Cholesterol concentration in bile was also measured. Results show that despite of process changes to ECF and waste water treatment improvements employed by the pulp and paper industry in the study area, it is still possible to detect elevated concentrations of these substances in the bile of fish exposed in the vici…
Occurrence of retene and resin acids in sediments and fish bile from a lake receiving pulp and paper mill effluents
Retene (7-isopropyl−1-methylphenanthrene) is a dialkyl-substituted PAH derived thermally and microbially from dehydroabietic acid. We have analyzed for retene and several resin acids in sediments at five depths at several sites in a lake receiving effluents from three pulp and paper mills, and two upstream reference sites. The highest concentration of retene was 1,600 μg/g dry weight (11,700 μg/g organic carbon [OC]) and of total resin acids was 1,500 μg/g dry weight (9,300 μg/g OC). Twelve kilometers downstream from the point of bleached-kraft mill effluent (BKME) discharge, the concentration of retene at a depth of 5 to 10 cm was 16 μg/g dry weight (650 μg/g OC) and of resin acids was 139…
BIOAVAILABILITY TO JUVENILE RAINBOW TROUT (ONCORYNCHUS MYKISS) OF RETENE AND OTHER MIXED-FUNCTION OXYGENASE-ACTIVE COMPOUNDS FROM SEDIMENTS
Retene (7-isopropyl-1-methylphenanthrene) is a naturally formed polycyclic aromatic hydrocarbon (PAH) that causes teratogenicity in fish larvae and induction of cytochrome P450 (CYP1A) enzymes. Retene occurs at high concentrations (or =3,300 microg/g dry wt) in surface sediments contaminated by resin acids from pulp mill effluents. To assess the environmental risks of retene, it is important to evaluate conditions affecting its bioavailability and accumulation by fish. Fingerling rainbow trout were exposed to retene-spiked or naturally contaminated sediments and sampled after 4 d to determine liver CYP1A activity and concentrations of retene metabolites in bile as indicators of retene accum…
Effects of primary- and secondary-treated bleached kraft mill effluents on the immune system and physiological parameters of roach.
The present study was designed to examine, whether, effluents from a modern pulp and paper mill using elemental chlorine-free/total chlorine-free (ECF/TCF) bleaching, exert effects on the immune system of fish and, in addition, to relate these findings to physiological parameters known to be affected by bleached kraft-mill effluents (BKME). Roach (Rutilus rutilus) were exposed in laboratory conditions to primary- or secondary-treated effluent from a pulp and paper mill. In order to study their capability to respond to foreign antigens they were immunised with bovine gamma-globulin (BGG) prior to exposure. The number of anti-BGG antibody-secreting cells (ASC) and the number of immunoglobulin…