0000000000050260

AUTHOR

Jennifer D. Oduro

showing 3 related works from this author

Peptide Processing Is Critical for T-Cell Memory Inflation and May Be Optimized to Improve Immune Protection by CMV-Based Vaccine Vectors.

2016

Cytomegalovirus (CMV) elicits long-term T-cell immunity of unparalleled strength, which has allowed the development of highly protective CMV-based vaccine vectors. Counterintuitively, experimental vaccines encoding a single MHC-I restricted epitope offered better immune protection than those expressing entire proteins, including the same epitope. To clarify this conundrum, we generated recombinant murine CMVs (MCMVs) encoding well-characterized MHC-I epitopes at different positions within viral genes and observed strong immune responses and protection against viruses and tumor growth when the epitopes were expressed at the protein C-terminus. We used the M45-encoded conventional epitope HGI…

0301 basic medicineMuromegalovirusEpitopes T-LymphocyteCD8-Positive T-LymphocytesLymphocyte ActivationPathology and Laboratory MedicineBiochemistryEpitopeMass SpectrometryMiceWhite Blood Cells0302 clinical medicineAnimal CellsMedicine and Health SciencesCytotoxic T celllcsh:QH301-705.5Antigens ViralImmune ResponseStainingVaccines SyntheticbiologyT CellsCell StainingHerpesviridae InfectionsFlow CytometryRecombinant Proteins3. Good healthmedicine.anatomical_structureMedical MicrobiologyViral PathogensVirusesHuman CytomegalovirusCellular TypesPathogensResearch Articlelcsh:Immunologic diseases. AllergyHerpesvirusesT cellImmune CellsAntigen presentationImmunologyCytotoxic T cellsMajor histocompatibility complexResearch and Analysis MethodsMicrobiology03 medical and health sciencesViral ProteinsImmune systemAntigenVirologyGeneticsmedicineAnimalsAntigen-presenting cellMolecular Biology TechniquesMolecular BiologyMicrobial PathogensBlood CellsImmunodominant EpitopesOrganismsBiology and Life SciencesProteinsViral VaccinesCell BiologyVirology030104 developmental biologylcsh:Biology (General)Specimen Preparation and Treatmentbiology.proteinMutagenesis Site-DirectedParasitologylcsh:RC581-607PeptidesDNA virusesImmunologic Memory030215 immunologyChromatography LiquidCloningPLoS pathogens
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The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages

2016

The receptor-like protein tyrosine phosphatase CD45 is expressed on the surface of cells of hematopoietic origin and has a pivotal role for the function of these cells in the immune response. Here we report that following infection of macrophages with mouse cytomegalovirus (MCMV) the cell surface expression of CD45 is drastically diminished. Screening of a set of MCMV deletion mutants allowed us to identify the viral gene m42 of being responsible for CD45 down-modulation. Moreover, expression of m42 independent of viral infection upon retroviral transduction of the RAW264.7 macrophage cell line led to comparable regulation of CD45 expression. In immunocompetent mice infected with an m42 del…

0301 basic medicineMuromegalovirusGenes ViralvirusesCell MembranesFluorescent Antibody TechniqueNEDD4Protein tyrosine phosphatasePathology and Laboratory MedicineBiochemistryLigasesWhite Blood CellsMice0302 clinical medicineSpectrum Analysis TechniquesUbiquitinAnimal CellsMedicine and Health SciencesBiology (General)Regulation of gene expressionStainingMice Inbred BALB CbiologyChemistryCell StainingAntigens CD45Herpesviridae InfectionsHuman cytomegalovirusFlow Cytometry3. Good healthEnzymesSpectrophotometryMedical MicrobiologyViral PathogensViruses293T cellsCell linesHuman CytomegalovirusCytophotometryCellular TypesCellular Structures and OrganellesPathogensBiological culturesBIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti.Research ArticleGene Expression Regulation ViralHerpesvirusesMCMV ; m42 ; CD45QH301-705.5Immune CellsImmunologyImmunoblottingDown-RegulationResearch and Analysis MethodsMicrobiologyGene product03 medical and health sciencesVirologyGeneticsAnimalsHumansMolecular BiologyMicrobial PathogensBlood CellsMacrophagesHEK 293 cellsBIOMEDICINE AND HEALTHCARE. Basic Medical Sciences.OrganismsBiology and Life SciencesProteinsMembrane ProteinsProtein phosphatase 2Cell BiologyRC581-607Ubiquitin LigasesMolecular biologyViral Replication030104 developmental biologyHEK293 CellsRAW 264.7 CellsViral replicationSpecimen Preparation and Treatmentbiology.proteinEnzymologyLeukocyte Common AntigensParasitologyImmunologic diseases. AllergyDNA viruses030215 immunology
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Murine cytomegalovirus (CMV) infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection

2016

Cytomegalovirus (CMV) is a ubiquitous virus, causing the most common congenital infection in humans, yet a vaccine against this virus is not available. Experimental studies of immunity against CMV in animal models of infection, such as the infection of mice with mouse CMV (MCMV), have relied mainly on parenteral infection protocols, although the virus naturally transmits by mucosal routes via body fluids. To characterize the biology of infections by mucosal routes, we compared the kinetics of virus replication, latent viral load and CD8 T-cell responses in lymphoid organs upon experimental intranasal (targeting the respiratory tract) and intragastric (targeting the digestive tract) infectio…

0301 basic medicineMuromegalovirusMice 129 StrainCongenital cytomegalovirus infectionSpleenCD8-Positive T-LymphocytesBiologyVirus ReplicationVirus03 medical and health sciencesImmunityVirologyVirus latencymedicineAnimalsImmunity MucosalMice Inbred BALB CAnimal StructuresViral Loadmedicine.diseaseVirologyVirus Latency030104 developmental biologymedicine.anatomical_structureLymphatic systemViral replicationModels AnimalImmunologyFemaleViral load
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