0000000000051998

AUTHOR

E. De La Cueva

showing 3 related works from this author

Exosomes in NSCLC: Analysis of its cargo as a source of biomarkers

2019

Oncologybusiness.industryCancer researchMedicineHematologybusinessMicrovesiclesAnnals of Oncology
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MA04.09 Study of Exosomes in NSCLC for Biomarkers Searching

2019

Pulmonary and Respiratory MedicineOncologybusiness.industryCancer researchMedicinebusinessMicrovesiclesJournal of Thoracic Oncology
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Exosomes in NSCLC as a source of biomarkers

2019

Abstract Background Exosomes are small membranous vesicles (around 40-130 nm), that have been detected in different biological samples, that play a key role in NSCLC and being relevant in stem cell differentiation as well. The main objective of this study was to analyze the exosomes cargo from NSCLC cell cultures growth in monolayer (2D) and suspension conditions (3D, lung tumorespheres). Methods Cultures were established from NSCLC resected patients and cell lines. Exosomes isolation was performed by ultracentrifugation. Characterization was carried out by NTA, electron microscopy, immunoblot and flow cytometry. Mutational status of EGFR and RAS genes was analyzed by BEAMing dPCR. Transcri…

medicine.diagnostic_testbusiness.industryCellular differentiationCancerHematologymedicine.diseaseMicrovesiclesFlow cytometryTranscriptomeOncologymicroRNAGene expressionmedicineCancer researchStem cellbusiness
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