Recent Advances in Complex Networks Theories with Applications
Seeking the Important Nodes of Complex Networks in Product R&D Team Based on Fuzzy AHP and TOPSIS
Published version of article in the journal: Mathematical Problems in Engineering. Alo available from the publisher at: http://dx.doi.org/10.1155/2013/327592 Open Access How to seek the important nodes of complex networks in product research and development (R&D) team is particularly important for companies engaged in creativity and innovation. The previous literature mainly uses several single indicators to assess the node importance; this paper proposes a multiple attribute decision making model to tentatively solve these problems. Firstly, choose eight indicators as the evaluation criteria, four from centralization of complex networks: degree centrality, betweenness centrality, closeness…
Ca2+ signalling plays a role in celastrol‐mediated suppression of synovial fibroblasts of rheumatoid arthritis patients and experimental arthritis in rats
Background and purpose Celastrol exhibits anti-arthritic effects in rheumatoid arthritis (RA), but the role of celastrol-mediated Ca2+ mobilization in treatment of RA remains undefined. Here, we describe a regulatory role for celastrol-induced Ca2+ signalling in synovial fibroblasts of RA patients and adjuvant-induced arthritis (AIA) in rats. Experimental approach We used computational docking, Ca2+ dynamics and functional assays to study the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump (SERCA). In rheumatoid arthritis synovial fibroblasts (RASFs)/rheumatoid arthritis fibroblast-like synoviocytes (RAFLS), mechanisms of Ca2+ -mediated autophagy were analysed by histological, immunohis…
Zircon ages of the Bayankhongor ophiolite mélange and associated rocks: Time constraints on Neoproterozoic to Cambrian accretionary and collisional orogenesis in Central Mongolia
Abstract Central Mongolia is geologically characterized by close juxtaposition of an accreted oceanic terrane with an arc-microcontinent collision zone. We present new U–Pb zircon ages and geochemical data for the Bayankhongor ophiolite melange from the oceanic terrane and for a syenite porphyry pluton from the arc-microcontinent zone, providing critical constraints on the regional evolution in late Neoproterozoic to early Cambrian times. An anorthosite (655 ± 4 Ma) associated with layered gabbro, a rodingite (metasomatized layered gabbro) (647 ± 6 Ma), and a high-level isotropic amphibole gabbro (647 ± 7 Ma) yielded the oldest zircon ages for the plutonic part of the ophiolite. A plagiogra…
Information Sharing and Channel Construction of Supply Chain under Asymmetric Demand Information
Information sharing and marketing channel building have become an important problem of supply chain management theory and practice. The research of information sharing focused on traditional channel of supply chain between upstream and downstream enterprises; however, the research ignores the behavior of information sharing with potential entrants and composite structure characteristics about traditional marketing channel with the direct channel. This paper uses the model to research the effects brought about sharing demand information with potential entrants and building marketing channel, which reveals information sharing and channel building mechanism in the supply chain. The study found…
Efficient Shielding of Polyplexes Using Heterotelechelic Polysarcosines
Shielding agents are commonly used to shield polyelectrolyte complexes, e.g. polyplexes, from agglomeration, precipitation in complex media, like blood, and thus enhance their circulation times in vivo. Since up to now primarily poly(ethylene glycol) (PEG) has been investigated to shield non-viral carriers for systemic delivery, we report on the use of polysarcosine (pSar) as a potential alternative for steric stabilization. A redox-sensitive, cationizable lipo-oligomer structure (containing two cholanic acids attached via a bioreducible disulfide linker to an oligoaminoamide backbone in T-shape configuration) was equipped with azide-functionality by solid phase supported synthesis. After m…
Devonian to Permian plate tectonic cycle of the Paleo-Tethys Orogen in southwest China (I): Geochemistry of ophiolites, arc/back-arc assemblages and within-plate igneous rocks
Abstract The Paleo-Tethys Orogen in southwest China is an amalgamation of continental terranes (Sibumasu, Simao and Yangtze), which are bounded by ophiolite belts (Ailaoshan, Jinshajiang and Changning–Menglian) that represent former ocean basins. This study concentrates on ophiolites, arc/back-arc assemblages, and within-plate igneous rocks that occur in this complex orogen. Mainly based on geochemical fingerprinting of basalts, the Paleo-Tethys ophiolites are here classified as MORB and SSZ types. The Ailaoshan ophiolite (NMORB-type; ca. 387–374 Ma) is associated with a non-volcanic segment of the rifted western Yangtze margin. The Jinshajiang ophiolite (EMORB-type; 346–341 Ma), which has …
Expression of semaphorin 3A and neuropilin 1 with clinicopathological features and survival in human tongue cancer
Objective: To investigate the association between semaphorin 3A (SEMA 3A) and its receptor neuropilin 1 (NRP1) and the clinicopathologic characteristics of patients with tongue cancer. Study Design: Forty-three tongue squamous cell carcinoma specimens were included. Immunohistochemical staining of SEMA3A and NRP1 was performed on 15 normal tongue epithelium specimens and the 43 tumour specimens. Immunoreactivity was evaluated based on the staining intensity and distribution score. Statistical analyses were performed using Chi-squared and Spearman tests and Kaplan-Meier analysis. Results: SEMA3A was significantly down-regulated in tongue cancer compared with normal tongue (P=0.025), while NR…
Key nodes of a microRNA network associated with the integrated mesenchymal subtype of high-grade serous ovarian cancer
Metastasis is the main cause of cancer mortality. One of the initiating events of cancer metastasis of epithelial tumors is epithelial-to-mesenchymal transition (EMT), during which cells dedifferentiate from a relatively rigid cell structure/morphology to a flexible and changeable structure/morphology often associated with mesenchymal cells. The presence of EMT in human epithelial tumors is reflected by the increased expression of genes and levels of proteins that are preferentially present in mesenchymal cells. The combined presence of these genes forms the basis of mesenchymal gene signatures, which are the foundation for classifying a mesenchymal subtype of tumors. Indeed, tumor classifi…
Cambrian ophiolite complexes in the Beishan area, China, southern margin of the Central Asian Orogenic Belt
Abstract We present zircon ages and geochemical data for Cambrian ophiolite complexes exposed in the Beishan area at the southern margin of the Central Asian Orogenic Belt (CAOB). The complexes consist of the Xichangjing-Xiaohuangshan and Hongliuhe-Yushishan ophiolites, which both exhibit complete ophiolite stratigraphy: chert, basalt, sheeted dikes, gabbro, mafic and ultramafic cumulates and serpentinized mantle peridotites. Zircon grains of gabbro samples yielded 206 Pb/ 238 U ages of 516 ± 8, 521 ± 4, 528 ± 3 and 535 ± 6 Ma that reflect the timing of gabbro emplacement. The geochemical data of the basaltic rocks show enrichment in large-ion lithophile elements and depletion in the high f…
SERCA and P-glycoprotein inhibition and ATP depletion are necessary for celastrol-induced autophagic cell death and collateral sensitivity in multidrug-resistant tumor cells.
Multidrug resistance (MDR) represents an obstacle in anti-cancer therapy. MDR is caused by multiple mechanisms, involving ATP-binding cassette (ABC) transporters such as P-glycoprotein (P-gp), which reduces intracellular drug levels to sub-therapeutic concentrations. Therefore, sensitizing agents retaining effectiveness against apoptosis- or drug-resistant cancers are desired for the treatment of MDR cancers. The sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) pump is an emerging target to overcome MDR, because of its continuous expression and because the calcium transport function is crucial to the survival of tumor cells. Previous studies showed that SERCA inhibitors exhibit anti-c…
Time scale of an early to mid-Paleozoic orogenic cycle of the long-lived Central Asian Orogenic Belt, Inner Mongolia of China: Implications for continental growth
Abstract We present a detailed, new time scale for an orogenic cycle (oceanic accretion–subduction–collision) that provides significant insights into Paleozoic continental growth processes in the southeastern segment of the long-lived Central Asian Orogenic Belt (CAOB). The most prominent tectonic feature in Inner Mongolia is the association of paired orogens. A southern orogen forms a typical arc-trench complex, in which a supra-subduction zone ophiolite records successive phases during its life cycle: birth (ca. 497–477 Ma), when the ocean floor of the ophiolite was formed; (2) youth (ca. 473–470 Ma), characterized by mantle wedge magmatism; (3) shortly after maturity (ca. 461–450 Ma), hi…
Correction to: Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study (Intensive Care Medicine, (2021), 47, 2, (160-169), 10.1007/s00134-020-06234-9)
The original version of this article unfortunately contained a mistake. The members of the ESICM Trials Group Collaborators were not shown in the article but only in the ESM. The full list of collaborators is shown below. The original article has been corrected.
Observation of the proton emitter 116,57La59
The quantum tunneling and emission of a single constituent nucleon provide a beautifully simple and unique window into the complex properties of atomic nuclei at the extreme edge of nuclear existence. In particular, for odd-odd proton emitting nuclides, the associated decay energy and partial half-life can be used to probe the correlations between the valence neutrons and protons which have been theoretically predicted to favour a new type of nuclear superfluidity, isoscalar neutron-proton pairing, for which the experimental “smoking gun" remains elusive. In the present work, proton emission from the lanthanum isotope 11657La59, 23 neutrons away from the only stable isotope 13957La82, is re…
Weaning from mechanical ventilation in intensive care units across 50 countries (WEAN SAFE)
Background: Current management practices and outcomes in weaning from invasive mechanical ventilation are poorly understood. We aimed to describe the epidemiology, management, timings, risk for failure, and outcomes of weaning in patients requiring at least 2 days of invasive mechanical ventilation. Methods: WEAN SAFE was an international, multicentre, prospective, observational cohort study done in 481 intensive care units in 50 countries. Eligible participants were older than 16 years, admitted to a participating intensive care unit, and receiving mechanical ventilation for 2 calendar days or longer. We defined weaning initiation as the first attempt to separate a patient from the ventila…
Carboniferous and Cretaceous mafic–ultramafic massifs in Inner Mongolia (China): A SHRIMP zircon and geochemical study of the previously presumed integral “Hegenshan ophiolite”
Abstract We present SHRIMP zircon ages and whole-rock geochemical data to identify mafic–ultramafic massifs of early Carboniferous and early Cretaceous ages from northern Inner Mongolia, China. The early Carboniferous massifs (Chongenshan–Xiaobaliang–Wusnihei) comprise predominantly lherzolite and minor gabbroic rocks. Zircons from a microgabbro (e Nd (t) = 9.8) and a plagiogranite (e Nd (t) = 9) yielded weighted mean 206 Pb/ 238 U ages of 354 ± 7 Ma and 333 ± 4 Ma, that we interpret as reflecting the time of zircon (and magma) crystallization. A volcanic breccia resting unconformably on the ultramafic rocks (serpentinites) has an eruption age of 300 ± 2 Ma and provides a younger minimum …
Episodic mantle melting-crustal reworking in the late Neoarchean of the northwestern North China Craton: Zircon ages of magmatic and metamorphic rocks from the Yinshan Block
Abstract The Yinshan Block in the northwestern North China Craton comprises late Neoarchean greenstones, high-grade metamorphic rocks (granulite, charnockite, enderbite and amphibole gneiss), diorites (sanukitoid and Mg-adakite) and granitoids (predominantly trondhjemite-tonalite). A greenstone terrane comprises a volcanic sequence of predominant basalt and dacite, with minor andesite. Zircons from a high-Mg andesite and an andesite have weighted mean 207Pb/206Pb ages of 2533 ± 5 Ma and 2510 ± 7 Ma, respectively, which reflect the timing of two distinct volcanic episodes. These two either predated or postdated a major volcanic episode that formed a basalt-dacite bimodal suite during 2515 ± …
Zircon ages and Hf isotopic compositions of Ordovician and Carboniferous granitoids from central Inner Mongolia and their significance for early and late Paleozoic evolution of the Central Asian Orogenic Belt
Abstract We present zircon ages and Hf-in-zircon isotopic data for plutonic rocks and review the evolution of central Inner Mongolia, China, in the early and late Paleozoic. Zircons of a granodiorite yielded a 206 Pb/ 238 U age of 472 ± 3 Ma that reflects the time of early Paleozoic magmatism. Zircon ages were also obtained for a tonalite (329 ± 3 Ma), quartz-diorite (320 ± 3 Ma), and granite vein (297 ± 2 Ma). Our results, in combination with published zircon ages and geochemical data, document distinct magmatic episodes in central Inner Mongolia. The dated samples are mostly granodiorite, tonalite and quartz-diorite in composition with intermediate to high-silica, high Na 2 O (3.08–4.26 w…
Evolution of a Permian intraoceanic arc–trench system in the Solonker suture zone, Central Asian Orogenic Belt, China and Mongolia
Abstract The identification of a fossil arc–trench system from the ophiolite-decorated Solonker suture zone in the southernmost Central Asian Orogenic Belt (CAOB) enables us to constrain the timing of pre-subduction extension (ca. 299–290 Ma), subduction initiation (ca. 294–280 Ma), ridge–trench collision (ca. 281–273 Ma) and slab break-off (ca. 255–248 Ma) in the Permian. A fraction of proto-arc crust (ca. 45 km long, up to 8 km wide) is preserved as a volcanic–plutonic sequence and is juxtaposed against a wide (ca. 30–80 km) forearc melange. This proto-arc crust comprises two distinct magma series, island arc tholeiite (IAT) and mid-ocean ridge basalt (MORB), both of which have strong sup…
Devonian to Permian plate tectonic cycle of the Paleo-Tethys Orogen in southwest China (II): Insights from zircon ages of ophiolites, arc/back-arc assemblages and within-plate igneous rocks and generation of the Emeishan CFB province
Abstract New SHRIMP zircon ages for ophiolites, arc/back-arc assemblages and within-plate igneous rocks constrain the evolution of the Paleo-Tethys Orogen of southwest China. Ophiolites represent remnants of the Paleo-Tethys ocean, which was divided by the Simao terrane into two tracts, the main ocean in the west (current position) and a branch in the east. The main ocean has been inferred to originate from Gondwana break-up in the Early Devonian, and the heritage is preserved as the Changning-Menglian ophiolite (SSZ-type). The oceanic branch is represented by the Ailaoshan (NMORB-type)–Jinshajinag (EMORB-type) ophiolites. Zircons from a diabase and a plagiogranite of the Ailaoshan ophiolit…
Magmatic and metamorphic development of an early to mid-Paleozoic continental margin arc in the southernmost Central Asian Orogenic Belt, Inner Mongolia, China
The Bainaimiao arc in Inner Mongolia, China, comprises a weakly metamorphosed volcani-sedimentary sequence and alow-P/Tmetamorphic complex.In this study wepresent SHRIMP zircon ages and geochemical data to document the temporal and genetic relationships between these two tectonic units. Zircons from a rhyolite and two dacites (high-K calc-alkaline) of the volcani-sedimentary sequence yielded 206 Pb/ 238
Attachment of Marine Sponge Cells of Hymeniacidon perleve on Microcarriers
Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were studied. Mixed cell population and enriched cell fractions of specific cell types by Ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. Cell attachment ratio (defined as the ratio of cells attached on microcarrier to the total number of cells in the culture) on glass beads is much higher than that on Cytodex 3 and Hillex for both mixed cell population and cell fraction at Ficoll 15-20% interface. The h…
Diagnosis and Classification of 17 Diseases from 1404 Subjects via Pattern Analysis of Exhaled Molecules.
We report on an artificially intelligent nanoarray based on molecularly modified gold nanoparticles and a random network of single-walled carbon nanotubes for noninvasive diagnosis and classification of a number of diseases from exhaled breath. The performance of this artificially intelligent nanoarray was clinically assessed on breath samples collected from 1404 subjects having one of 17 different disease conditions included in the study or having no evidence of any disease (healthy controls). Blind experiments showed that 86% accuracy could be achieved with the artificially intelligent nanoarray, allowing both detection and discrimination between the different disease conditions examined.…
Zircon ages and Hf isotopic compositions of plutonic rocks from the Central Tianshan (Xinjiang, northwest China) and their significance for early to mid-Palaeozoic crustal evolution
We present new zircon ages and Hf-in-zircon isotopic data for plutonic rocks and review the crustal evolution of the Chinese Central Tianshan (Xinjiang, northwest China) in the early to mid-Palaeozoic. The Early Ordovician (ca. 475–473 Ma) granitoid rocks have zircon eHf(t) values either positive (+0.3 to +9.5) or negative (−6.0 to −12.9). This suggests significant addition of juvenile material to, and coeval crustal reworking of, the pre-existing continental crust that is fingerprinted by numerous Precambrian zircon xenocrysts. The Late Ordovician–Silurian (ca. 458–425 Ma) rocks can be assigned to two sub-episodes of magmatism: zircon from rocks of an earlier event (ca. 458–442 Ma) has neg…
Collaborative Development Planning Model of Supporting Product in Platform Innovation Ecosystem
Published version of an article in the journal: Mathematical Problems in Engineering. Also available from the publisher at: http://10.1155/2014/690589 In order to improve the market value of the product, the platform enterprise often participates in the development process of supporting product of emerging industry's platform innovation ecosystem. This paper puts forward a revenue sharing contract between the platform company and the supporting company by creating a collaborative development model of the supporting product in the ecosystem, and this paper studies the platform enterprise investment resource property's (complementary or substitution) impact on the supporting enterprise R&D ef…
Zircon dating of Neoproterozoic and Cambrian ophiolites in West Mongolia and implications for the timing of orogenic processes in the central part of the Central Asian Orogenic Belt
Abstract We present new isotopic and trace element data to review the geochronological/geochemical/geological evolution of the central part of the Central Asian Orogenic Belt (CAOB), and find a fundamental geological problem in West Mongolia, which has traditionally been subdivided into northwestern early Paleozoic (formerly Caledonian) and southerly late Paleozoic (formerly Hercynian) belts by the Main Mongolian Lineament (MML). We resolve this problem with SHRIMP zircon dating of ophiolites and re-evaluation of much published literature. In Northwest Mongolia the Dariv–Khantaishir ophiolite marks the boundary between the Lake arc in the west and the Dzabkhan–Baydrag microcontinent in the …
Evolution Over Time of Ventilatory Management and Outcome of Patients With Neurologic Disease
OBJECTIVES: To describe the changes in ventilator management over time in patients with neurologic disease at ICU admission and to estimate factors associated with 28-day hospital mortality. DESIGN: Secondary analysis of three prospective, observational, multicenter studies. SETTING: Cohort studies conducted in 2004, 2010, and 2016. PATIENTS: Adult patients who received mechanical ventilation for more than 12 hours. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Among the 20,929 patients enrolled, we included 4,152 (20%) mechanically ventilated patients due to different neurologic diseases. Hemorrhagic stroke and brain trauma were the most common pathologies associated with the need fo…
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Contains fulltext : 232759.pdf (Publisher’s version ) (Closed access) In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to…
Application of a MTT Assay for Screening Nutritional Factors in Growth Media of Primary Sponge Cell Culture
Marine sponges (Porifera) are producers of the largest variety of bioactive compounds among benthic marine organisms. In vitro culture of marine sponge cells has been proposed for the sustainable production of these pharmacologically interesting compounds from marine sponges but with limited success. The development of a suitable growth medium is an essential prerequisite for sponge cells grown in vitro. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was adapted to screen for potential nutritional factors in formulating a growth medium for primary cell culture of Suberites domuncula. In 96-well plates, the effects of nutritional factors including glutamine, pyr…
Autophagy
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide…
Age and provenance constraints on seismically-determined crustal layers beneath the Paleozoic southern Central Asian Orogen, Inner Mongolia, China
Abstract We present 110 ages and 51 in-situ δ18O values for zircon xenocrysts from a post-99 Ma intraplate basaltic rock suite hosted in a subduction–accretion complex of the southern Central Asian Orogenic Belt in order to constrain a seismic profile across the Paleozoic Southern Orogen of Inner Mongolia and the northern margin of the North China Craton. Two zircon populations are recognized, namely a Phanerozoic group of 70 zircons comprising granitoid-derived (ca. 431–99 Ma; n = 31; peak at 256 Ma), meta-granitoid-derived (ca. 449–113 Ma; n = 24; peak at 251 Ma) and gabbro-derived (436–242 Ma; n = 15; peaks at 264 and 244 Ma) grains. Each textural type is characterized by a distinct zirc…
Functional FLT1 genetic variation is a prognostic factor for recurrence in stage I-III non-small cell lung cancer
Background: We propose that single-nucleotide polymorphisms (SNPs) in genes of the vascular endothelial growth factor pathway of angiogenesis will associate with survival in non-small-cell lung cancer (NSCLC) patients. Methods: Fifty-three SNPs in vascular endothelial growth factor-pathway genes were genotyped in 150 European stage I-III NSCLC patients and tested for associations with patient survival. Replication was performed in an independent cohort of 142 European stage I-III patients. Reporter gene assays were used to assess the effects of SNPs on transcriptional activity. Results: In the initial cohort, five SNPs associated (q < 0.05) with relapse-free survival (RFS). The minor allele…
Zircon ages of metamorphic and magmatic rocks within peridotite-bearing mélanges: Crucial time constraints on early Carboniferous extensional tectonics in the Chinese Tianshan
Abstract We dated and geochemically characterized peridotite-bearing melanges in the Chinese South Tianshan and within the Main Tianshan Shear Zone. The Yushugou-Tonghuashan melange in the Chinese South Tianshan exposes a tectonic juxtaposition of a diapirically emplaced metaperidotite (predominantly lherzolite) massif with a high-grade metamorphic terrane (ca. 10 km long; protolith age ≥ ca. 445–466 Ma). Metamorphic zircons of a mafic granulite (eNd(t) = 5.0) yielded a weighted mean 206Pb/238U age of 341 ± 8 Ma that we interpret as the time of granulite-facies metamorphism. The youngest zircon rims of an intermediate granulite (eNd(t) = − 4.3) have a mean age of 332 ± 13 Ma that records a …
Application of remote online learning in oral histopathology teaching in China
Background The aim of this study was to investigate the application of remote learning and virtual microscopy in oral histopathology teaching, a unique experience in China. The oral histopathology teaching in Nanjing Medical University has been extraordinary. Material and Methods 98 third-year dental students of Grade 2016 took oral histopathology theoretical course face-to-face in 2019 (Traditional group). The 94 participants of Grade 2017 took online oral histopathology course using digital methods(E-Learning platform and Virtual Simulation Experiment Teaching Center for Dentistry) in 2020. During the practical laboratory sessions, the students in both Traditional group and Online group o…
Zircon ages and Hf isotopic compositions of plutonic rocks from the Central Tianshan (Xinjiang, northwest China) and their significance for early to mid-Palaeozoic crustal evolution
We present new zircon ages and Hf-in-zircon isotopic data for plutonic rocks and review the crustal evolution of the Chinese Central Tianshan (Xinjiang, northwest China) in the early to mid-Palaeozoic. The Early Ordovician (ca. 475–473 Ma) granitoid rocks have zircon εHf(t) values either positive (+0.3 to +9.5) or negative (−6.0 to −12.9). This suggests significant addition of juvenile material to, and coeval crustal reworking of, the pre-existing continental crust that is fingerprinted by numerous Precambrian zircon xenocrysts. The Late Ordovician–Silurian (ca. 458–425 Ma) rocks can be assigned to two sub-episodes of magmatism: zircon from rocks of an earlier event (ca. 458–442 Ma) has neg…