0000000000054247
AUTHOR
Philipp Hühn
How challenging RADseq data turned out to favor coalescent-based species tree inference. A case study in Aichryson (Crassulaceae)
Analysing multiple genomic regions while incorporating detection and qualification of discordance among regions has become standard for understanding phylogenetic relationships. In plants, which usually have comparatively large genomes, this is feasible by the combination of reduced-representation library (RRL) methods and high-throughput sequencing enabling the cost effective acquisition of genomic data for thousands of loci from hundreds of samples. One popular RRL method is RADseq. A major disadvantage of established RADseq approaches is the rather short fragment and sequencing range, leading to loci of little individual phylogenetic information. This issue hampers the application of coa…
Salvia apiana — A carpenter bee flower?
Abstract Salvia apiana has unique mask flowers restricting access to nectar by a bulged lower lip. Stamens and style protrude the flower tube. Most interesting, the staminal lever mechanism usually characterizing bee flowers in Salvia L. is lacking. In the present study, we aim to understand the peculiar pollination mechanism and to identify the pollinators and breeding system of the species. Field experiments were conducted at three natural localities in Southern California and the Rancho Santa Ana Botanical Garden, Claremont. Pollinator behavior was documented on video and interpreted considering frequency, handling time, percentage of successfully touching pollen and stigma, pollen depos…