0000000000054813

AUTHOR

Klaus-michael Keller

showing 2 related works from this author

Detection of Hepatitis B Virus DNA in the Liver of Children with Chronic Hepatitis B by In Situ Hybridization and Its Relation to Other Viral Markers

1992

The aim of the study was to detect hepatitis B virus (HBV) DNA by in situ hybridization (ISH) with a 35S-labeled radioactive probe in frozen liver biopsy tissue sections of 63 hepatitis B virus surface antigen (HBsAg)-positive children. The results were compared to other markers of viral replication. HBV DNA was detected in 48 children. Of the 15 negative cases, four had hepatitis B envelope antigen (HBeAg), 10 anti-HBe, and one neither HBeAg nor anti-HBe. Free HBV DNA in serum and liver was positive in one patient. Forty of the positive children were HBeAg- and six anti-HBe-positive; two were negative for both. Of 45 36 had HBV DNA in serum. In 38 of 47 HBV DNA and in 31 of 42 HBcAg could …

Genetic MarkersMaleHepatitis B virusHBsAgAdolescentHepatitis B virus DNA polymerasemedicine.disease_causemedicineHumansChildHepatitis B virusbiologymedicine.diagnostic_testGastroenterologyInfantNucleic Acid Hybridizationvirus diseasesHepatitis BHepatitis Bbiology.organism_classificationmedicine.diseaseHepatitis B Core AntigensVirologydigestive system diseasesBlotting SouthernHBcAgLiverHepadnaviridaeHBeAgChild PreschoolLiver biopsyChronic DiseaseDNA ViralPediatrics Perinatology and Child HealthFemaleJournal of Pediatric Gastroenterology and Nutrition
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Hepatitis B Virus DNA in Liver Tissue of Chronic HBsAg Carriers in Childhood and Its Relationship to Other Viral Markers

1992

The aim of the study was to examine the state of hepatitis B virus (HBV) DNA in liver tissue of 103 children with chronic hepatitis B aged 0.5-18 years to detect free and integrated viral sequences by Southern blot hybridization. HBV DNA was found in 74 patients. Seventy-two were seropositive for hepatitis B e antigen (HBeAg) and two had anti-HBe antibodies. Integrated sequences could be demonstrated in two children. One of them had only integrated HBV DNA and was anti-HBe seropositive. The other one presented both free and integrated viral sequences and developed seroconversion from HBeAg to anti-HBe 5 months after biopsy. In 29 hepatitis B surface antigen (HBsAg) carriers, no HBV DNA coul…

MaleHepatitis B virusHBsAgAdolescentHepatitis B virus DNA polymerasemedicine.disease_causeHumansMedicineSeroconversionChildSouthern blotHepatitis B virusHepatitis B Surface Antigensbusiness.industryLiver cellGastroenterologyInfantvirus diseasesHepatitis BVirologydigestive system diseasesBlotting SouthernLiverHBeAgChild PreschoolCarrier StateChronic DiseaseDNA ViralPediatrics Perinatology and Child HealthImmunologyFemalebusinessViral loadJournal of Pediatric Gastroenterology and Nutrition
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