THE EFFECTS OF MATING SYSTEM AND GENETIC VARIABILITY ON SUSCEPTIBILITY TO TREMATODE PARASITES IN A FRESHWATER SNAIL, LYMNAEA STAGNALIS

The amount and distribution of genetic variability in host populations can have significant effects on the outcome of host-parasite interactions. We studied the effect of mating system and genetic variability on susceptibility of Lymnaea stagnalis snails to trematode parasites. Mating system of snails from eight populations differing in the amount of genetic variability was manipulated, and self- and cross-fertilized offspring were exposed to naturally occurring trematode parasites in a controlled lake experiment. Susceptibility of snails varied between populations, but mating-system treatment did not have a significant effect. Heterozygosity of snails was negatively correlated with the pro…

Does metal contamination affect clonal diversity of the parthenogenetic earthworm Dendrobaena octaedra?

Clonal diversity of the parthenogenetic earthworm Dendrobaena octaedra was studied in three metal contamination gradients in Finland. Metal concentrations in soils (both total and bioavailable fractions) and in earthworm tissues were analysed at each sampling site. Allozyme electrophoresis was used to determine clonal diversity and several genetic diversity measures were used to evaluate differences between populations at metal contaminated and uncontaminated sites. Cu and Zn in the soils and in the earthworms increased with decreasing distance from the emission sources in all areas. Metal contamination appeared to affect clonal diversity of D. octaedra only slightly, since clonal diversity…

Impact of genetic diversity of an earthworm on decomposition and ecosystem functioning

Abstract Ecosystem functioning is affected positively by increased biodiversity, through complementary functions of multiple species or because high-functioning species are more likely in a species-rich community. Genetic diversity is one level of biodiversity that has been shown to positively affect ecosystem functioning. Whether the genetic diversity of a key decomposer species affects decomposition processes, and ecosystem functioning in general, is still unknown. We compared low and high genetic diversity assemblages of the earthworm Dendrobaena octaedra in two different experiments: using microcosms containing a simple community of other decomposer animals (some nematodes and other mic…

Evolutionary conservation and function of the human embryonic stem cell specific miR-302/367 cluster

miRNA clusters define a group of related miRNAs closely localized in the genome with an evolution that remains poorly understood. The miR-302/367 cluster represents a single polycistronic transcript that produces five precursor miRNAs. The cluster is highly expressed and essential for maintenance of human embryonic stem cells. We found the cluster to be highly conserved and present in most mammals. In primates, seed sequence and miRNA structure are conserved, but inter-precursor sequences are evolving. Insertions of new miRNAs, deletions of individual miRNAs, and a cluster duplication observed in different species suggest an actively evolving cluster. Core transcriptional machinery consisti…

Morphological and molecular analysis of Henricia Gray, 1840 (Asteroidea: Echinodermata) from the Northern Atlantic Ocean

High maternal species density mediates unidirectional heterospecific matings inCalopteryxdamselflies

Hybridization is a well-known phenomenon, but there are still relatively few studies addressing the question of reproductive isolation between related sympatric animal species with largely overlapping ranges. Population density, relative abundance, and operational sex ratio (OSR) are among the factors known to have an influence on the frequency of heterospecific matings in sympatric populations. Here we had two aims. First, we used microsatellite markers and field observations to study the frequency of hybrids, and backcrosses, and the rate of heterospecific matings between two sympatric damselfly species Calopteryx splendens (Harris, 1780) and Calopteryx virgo (Linne, 1758). Second, we inv…

Species and genetic diversity relationships in benthic macroinvertebrate communities along a salinity gradient

Background Species- and genetic diversity can change in parallel, resulting in a species-genetic diversity correlation (SGDC) and raising the question if the same drivers influence both biological levels of diversity. The SGDC can be either positive or negative, depending on whether the species diversity and the genetic diversity of the measured species respond in the same or opposite way to drivers. Using a traditional species diversity approach together with ultra-conserved elements and high throughput sequencing, we evaluated the SGDCs in benthic macrofauna communities in the Baltic Sea, a geologically young brackish water sea characterised by its steep salinity gradient and low species …

Polymorphism in Developmental Mode and Its Effect on Population Genetic Structure of a Spionid Polychaete, Pygospio elegans

Population genetic structure of sedentary marine species is expected to be shaped mainly by the dispersal ability of their larvae. Long-lived planktonic larvae can connect populations through migration and gene flow, whereas species with nondispersive benthic or direct-developing larvae are expected to have genetically differentiated populations. Poecilogonous species producing different larval types are ideal when studying the effect of developmental mode on population genetic structure and connectivity. In the spionid polychaete Pygospio elegans, different larval types have been observed between, and sometimes also within, populations. We used microsatellite markers to study population st…

Systematics and population genetics of the coldwater (Etheostoma ditrema) and watercress (Etheostoma nuchale) darters, with comments on the Gulf darter (Etheostoma swaini) (Percidae: subgenus Oligocephalus)

Abstract Current taxonomy of the Etheostoma asprigene species group recognizes four species, two of which ( Etheostoma ditrema Ramsey and Suttkus and Etheostoma nuchale Howell and Caldwell) are restricted to isolated springs and spring-fed stream systems above the Fall Line of the Mobile Basin of Alabama, Georgia, and Tennessee. Previous studies of morphological and biochemical variation between disjunct populations of E. ditrema support the presence of multiple independent lineages. Unfortunately, the lack of phylogenetic methodology has made it impossible to distinguish historically differentiated non-sister taxa from currently intergrading sister lineages. The current study examines memb…

De novo transcriptome assembly and developmental mode specific gene expression of Pygospio elegans

Species with multiple different larval developmental modes are interesting models for the study of mechanisms underlying developmental mode transitions and life history evolution. Pygospio elegans, a small, tube-dwelling polychaete worm commonly found in estuarine and marine habitats around the northern hemisphere, is one species with variable developmental modes. To provide new genomic resources for studying P. elegans and to address the differences in gene expression between individuals producing offspring with different larval developmental modes, we performed whole transcriptome Illumina RNA sequencing of adult worms from two populations and prepared a de novo assembly of the P. elegans…

Seasonal genetic variation associated with population dynamics of a poecilogonous polychaete worm

Poecilogonous species show variation in developmental mode, with larvae that differ both morphologically and ecologically. The spionid polychaete Pygospio elegans shows variation in developmental mode not only between populations, but also seasonally within populations. We investigated the consequences of this developmental polymorphism on the spatial and seasonal genetic structure of P. elegans at four sites in the Danish Isefjord‐Roskilde‐Fjord estuary at six time points, from March 2014 until February 2015. We found genetic differentiation between our sampling sites as well as seasonal differentiation at two of the sites. The seasonal genetic shift correlated with the appearance of new s…

Temporal genetic structure in a poecilogonous polychaete: the interplay of developmental mode and environmental stochasticity

Background: Temporal variation in the genetic structure of populations can be caused by multiple factors, including natural selection, stochastic environmental variation, migration, or genetic drift. In benthic marine species, the developmental mode of larvae may indicate a possibility for temporal genetic variation: species with dispersive planktonic larvae are expected to be more likely to show temporal genetic variation than species with benthic or brooded non-dispersive larvae, due to differences in larval mortality and dispersal ability. We examined temporal genetic structure in populations of Pygospio elegans, a poecilogonous polychaete with within-species variation in developmental m…

De novo transcriptome assembly of a facultative parasitic nematode Pelodera (syn. Rhabditis) strongyloides

Pelodera strongyloides is a generally free-living gonochoristic facultative nematode. The whole genomic sequence of P. strongyloides remains unknown but 4 small subunit ribosomal RNA (ssrRNA) gene sequences are available. This project launched a de novo transcriptome assembly with 100 bp paired-end RNA-seq reads from normal, starved and wet-plate cultured animals. Trinity assembly tool generated 104,634 transcript contigs with N50 contig being 2195 bp and average contig length at 1103 bp. Transcriptome BLASTX matching results of five nematodes (C. elegans, Strongyloides stercoralis, Necator americanus, Trichuris trichiura, and Pristionchus pacificus) were consistent with their evolutionary …

DNA Methylation and Potential for Epigenetic Regulation in Pygospio elegans.

Transitions in developmental mode are common evolutionarily, but how and why they occur is not understood. Developmental mode describes larval phenotypes, including morphology, ecology and behavior of larvae, which typically are generalized across different species. The polychaete worm Pygospio elegans is one of few species polymorphic in developmental mode, with multiple larval phenotypes, providing a possibility to examine the potential mechanisms allowing transitions in developmental mode. We investigated the presence of DNA methylation in P. elegans, and, since maternal provisioning is a key factor determining eventual larval phenotype, we compared patterns of DNA methylation in females…

Population and reproductive dynamics of the polychaete Pygospio elegans in a boreal estuary complex

Pygospio elegans is an opportunistic, wide-spread spionid polychaete that reproduces asexually via fragmentation and can produce benthic and pelagic larvae, hence combining different developmental modes in one species. We documented the density, size distribution, and reproductive activity of P. elegans at four sites in the Danish Isefjord-Roskilde Fjord estuary complex, where all modes of reproduction were reported. We compared population dynamics of this species to environmental parameters such as salinity, temperature, and sediment characteristics (grain size, sorting, porosity, water content, organic content, C/N). We observed that new cohorts—resulting either from sexual or asexual rep…

GENETIC VARIABILITY AND DRIFT LOAD IN POPULATIONS OF AN AQUATIC SNAIL

Population genetic theory predicts that in small populations, random genetic drift will fix and accumulate slightly deleterious mutations, resulting in reduced reproductive output. This genetic load due to random drift (i.e., drift load) can increase the extinction risk of small populations. We studied the relationship between genetic variability (indicator of past population size) and reproductive output in eight isolated, natural populations of the hermaphroditic snail Lymnaea stagnalis. In a common laboratory environment, snails from populations with the lowest genetic variability mature slower and have lower fecundity than snails from genetically more variable populations. This result s…

Predominance of outcrossing in Lymnaea stagnalis despite low apparent fitness costs of self-fertilization.

We have quantified the natural mating system in eight populations of the simultaneously hermaphroditic aquatic snail Lymnaea stagnalis, and studied the ecological and genetic forces that may be directing mating system evolution in this species. We investigated whether the natural mating system can be explained by the availability of mates, by the differential survival of self- and cross-fertilized snails in nature, and by the effects of mating system on parental fecundity and early survival. The natural mating system of L. stagnalis was found to be predominantly cross-fertilizing. Density of snails in the populations had no relationship with the mating system, suggesting that outcrossing ra…

Acute and chronic response to a change in salinity of the euryhaline polychaete Pygospio elegans (Claparède)

Abstract Estuaries are thought to be very harsh environments because their physico-chemical parameters, such as salinity, temperature and oxygen, can fluctuate substantially. The distribution of species living in an estuary is largely determined by how well they can cope with such fluctuations. The spionid polychaete Pygospio elegans is common in boreal estuarine habitats that show strong salinity fluctuations, living in habitats ranging from fully marine salinities to brackish environments with salinities as low as 5. In this study we investigated the abilities of P. elegans to cope with an acute as well as a long-term change in salinity. Specimens originating from a salinity of about 15 w…

Interspecific interactions influence contrasting spatial genetic structures in two closely related damselfly species

Spatial genetic structure (SGS) is largely determined by colonization history, landscape and ecological characteristics of the species. Therefore, sympatric and ecologically similar species are expected to exhibit similar SGSs, potentially enabling prediction of the SGS of one species from that of another. On the other hand, due to interspecific interactions, ecologically similar species could have different SGSs. We explored the SGSs of the closely related Calopteryx splendens and Calopteryx virgo within Finland and related the genetic patterns to characteristics of the sampling localities. We observed different SGSs for the two species. Genetic differentiation even within short distances …

Low Levels of Mitochondrial DNA and Symbiont Diversity in the Worldwide Agricultural Pest, the Greenhouse Whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)

Trialeurodes vaporariorum, the greenhouse whitefly, is a cosmopolitan agricultural pest. Little is known about the genetic diversity of T. vaporariorum and the bacterial symbionts associated with this species. Here, we undertook a large phylogeographic study by investigating both the mitochondrial (mt) diversity and the infection status of 38 T. vaporariorum collections from 18 countries around the world. Genetic diversity of T. vaporariorum was studied by analyzing sequence data from the mt cytochrome oxidase I, cytochrome b, and NADH dehydrogenase subunit 5 genes. Maximum-likelihood (ML) phylogeny reconstruction delineated 2 clades characterized by limited sequence divergence: one clade c…

First record of Beroe gracilis Künne, 1939 (Ctenophora: Beroida: Beroidae) from Norway, found in a Mnemiopsis leidyi A. Agassiz, 1865 bloom

In September 2014 an unusual mixture of ctenophores was recorded at Arboretet, south-western Norway and at Flødevigen, near Arendal on the south coast of Norway. In addition to the invasive American lobate ctenophore,Mnemiopsis leidyi, the common northern lobate ctenophoreBolinopsis infundibulumand the cydippidPleurobrachia pileus, two beroid ctenophores, were noted –Beroe cucumisandBeroegracilis. The latter species had not been documented before in Norwegian waters.

What do we need to know about speciation?

Speciation has been a major focus of evolutionary biology research in recent years, with many important advances. However, some of the traditional organising principles of the subject area no longer provide a satisfactory framework, such as the classification of speciation mechanisms by geographical context into allopatric, parapatric and sympatry classes. Therefore, we have asked where speciation research should be directed in the coming years. Here, we present a distillation of questions about the mechanisms of speciation, the genetic basis of speciation and the relationship between speciation and diversity. Our list of topics is not exhaustive; rather we aim to promote discussion on rese…

Variation in gene expression within clones of the earthworm Dendrobaena octaedra

Gene expression is highly plastic, which can help organisms to both acclimate and adapt to changing environments. Possible variation in gene expression among individuals with the same genotype (among clones) is not widely considered, even though it could impact the results of studies that focus on gene expression phenotypes, for example studies using clonal lines. We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. Five microsatellite markers were developed and used to confirm that offspring are genetic clones of their parent. After that, expression of 12 genes was measured…

miRToolsGallery: a tag-based and rankable microRNA bioinformatics resources database portal

Abstract Hundreds of bioinformatics tools have been developed for MicroRNA (miRNA) investigations including those used for identification, target prediction, structure and expression profile analysis. However, finding the correct tool for a specific application requires the tedious and laborious process of locating, downloading, testing and validating the appropriate tool from a group of nearly a thousand. In order to facilitate this process, we developed a novel database portal named miRToolsGallery. We constructed the portal by manually curating > 950 miRNA analysis tools and resources. In the portal, a query to locate the appropriate tool is expedited by being searchable, filterable and …

Introduction to Symposium: Poecilogony--A Window on Larval Evolutionary Transitions in Marine Invertebrates

Poecilogony is the intraspecific variation in developmental mode that has been described in some marine invertebrates. Poecilogonous species produce different larval forms (e.g., free-swimming planktotrophic larvae as well as brooded lecithotrophic or adelphophagic larvae). Poecilogony can be a controversial topic, since it is difficult to identify and characterize the phenomenon with certainty. It has been challenging to determine whether poecilogony represents developmental polymorphism with a genetic basis or developmental polyphenism reflecting plastic responses to environmental cues. Other outstanding questions include whether common mechanisms underlie the developmental variation we o…

Data from: Temporal genetic structure in a poecilogonous polychaete: the interplay of developmental mode and environmental stochasticity

Background: Temporal variation in the genetic structure of populations can be caused by multiple factors, including natural selection, stochastic environmental variation, migration, or genetic drift. In benthic marine species, the developmental mode of larvae may indicate a possibility for temporal genetic variation: species with dispersive planktonic larvae are expected to be more likely to show temporal genetic variation than species with benthic or brooded non-dispersive larvae, due to differences in larval mortality and dispersal ability. We examined temporal genetic structure in populations of Pygospio elegans, a poecilogonous polychaete with within-species variation in developmental m…

Data from: The effect of inbreeding rate on fitness, inbreeding depression and heterosis over a range of inbreeding coefficients

Understanding the effects of inbreeding and genetic drift within populations and hybridization between genetically differentiated populations is important for many basic and applied questions in ecology and evolutionary biology. The magnitudes and even the directions of these effects can be influenced by various factors, especially by the current and historical population size (i.e., inbreeding rate). Using Drosophila littoralis as a model species, we studied the effect of inbreeding rate over a range of inbreeding levels on 1) mean fitness of a population (relative to that of an outbred control population), 2) within-population inbreeding depression (reduction in fitness of offspring from …

Data from: Seasonal genetic variation associated with population dynamics of a poecilogonous polychaete worm

Poecilogonous species show variation in developmental mode, with larvae that differ both morphologically and ecologically. The spionid polychaete Pygospio elegans shows variation in developmental mode not only between populations, but also seasonally within populations. We investigated the consequences of this developmental polymorphism on the spatial and seasonal genetic structure of P. elegans at four sites in the Danish Isefjord-Roskilde-Fjord estuary at six time points, from March 2014 until February 2015. We found genetic differentiation between our sampling sites as well as seasonal differentiation at two of the sites. The seasonal genetic shift correlated with the appearance of new s…

Data from: DNA methylation and potential for epigenetic regulation in Pygospio elegans

Transitions in developmental mode are common evolutionarily, but how and why they occur is not understood. Developmental mode describes larval phenotypes, including morphology, ecology and behavior of larvae, which typically are generalized across different species. The polychaete worm Pygospio elegans is one of few species polymorphic in developmental mode, with multiple larval phenotypes, providing a possibility to examine the potential mechanisms allowing transitions in developmental mode. We investigated the presence of DNA methylation in P. elegans, and, since maternal provisioning is a key factor determining eventual larval phenotype, we compared patterns of DNA methylation in females…

Data from: Interspecific interactions influence contrasting spatial genetic structures in two closely related damselfly species

Spatial genetic structure (SGS) is largely determined by colonization history, landscape and ecological characteristics of the species. Therefore, sympatric and ecologically similar species are expected to exhibit similar SGSs, potentially enabling prediction of the SGS of one species from that of another. On the other hand, due to interspecific interactions, ecologically similar species could have different SGSs. We explored the SGSs of the closely related Calopteryx splendens and C. virgo within Finland and related the genetic patterns to characteristics of the sampling localities. We observed different SGSs for the two species. Genetic differentiation even within short distances in C. sp…