0000000000059290
AUTHOR
Beatriz Vallejo
Análisis de las rutas de señalización de nutrientes en cepas vínicas de Saccharomyces cerevisiae en condiciones de vinificación
Tesis doctoral presentada por Beatriz Vallejo Estará para optar al grado de Doctora por la Universitat de València.
Herbicide glufosinate inhibits yeast growth and extends longevity during wine fermentation.
Glufosinate ammonium (GA) is a widely used herbicide that inhibits glutamine synthetase. This inhibition leads to internal amino acid starvation which, in turn, causes the activation of different nutrient sensing pathways. GA also inhibits the enzyme of the yeast Saccharomyces cerevisiae in such a way that, although it is not used as a fungicide, it may alter yeast performance in industrial processes like winemaking. We describe herein how GA indeed inhibits the yeast growth of a wine strain during the fermentation of grape juice. In turn, GA extends longevity in a variety of growth media. The biochemical analysis indicates that GA partially inhibits the nutrient sensing TORC1 pathway, whic…
Role of saccharomyces cerevisiae nutrient signaling pathways during winemaking: a phenomics approach
The ability of the yeast Saccharomyces cerevisiae to adapt to the changing environment of industrial processes lies in the activation and coordination of many molecular pathways. The most relevant ones are nutrient signaling pathways because they control growth and stress response mechanisms as a result of nutrient availability or scarcity and, therefore, leave an ample margin to improve yeast biotechnological performance. A standardized grape juice fermentation assay allowed the analysis of mutants for different elements of many nutrient signaling pathways under different conditions (low/high nitrogen and different oxygenation levels) to allow genetic-environment interactions to be analyze…