0000000000059937
AUTHOR
Rudolf Beyer
Is “I-DNA” derived from Nuclear DNA ?
On the basis of double radioactive labelling and buoyant density studies, it is concluded that “I-DNA” is not a separate entity from nuclear DNA but may be an artefact derived from it.
Highly protective alkalinization by ammonia vapor diffusion in viscosimetric DNA damage assessment.
A method for the measurement of viscosities correlated to DNA alterations in alkaline homogenate suspensions is described. The alkaline pH shift to afford cell lysis, DNA unfolding, and denaturation is attained by gaseous ammonia diffusion, thus avoiding shear stress from mechanical mixing. At the same time a stabilizing density gradient is established. This solution is run through a plastic measuring tube that is wide enough to minimize the influence of uneven swelling of the lysing DNA-containing components. Flow times under a carefully controlled water head are registered, and their ratios to control solutions are evaluated. The relative viscosities show a strong and irreversible depende…