0000000000064641

AUTHOR

Wolf D. Lehmann

showing 2 related works from this author

Model Based Targeting of IL-6-Induced Inflammatory Responses in Cultured Primary Hepatocytes to Improve Application of the JAK Inhibitor Ruxolitinib

2017

IL-6 is a central mediator of the immediate induction of hepatic acute phase proteins (APP) in the liver during infection and after injury, but increased IL-6 activity has been associated with multiple pathological conditions. In hepatocytes, IL-6 activates JAK1-STAT3 signaling that induces the negative feedback regulator SOCS3 and expression of APPs. While different inhibitors of IL-6-induced JAK1-STAT3-signaling have been developed, understanding their precise impact on signaling dynamics requires a systems biology approach. Here we present a mathematical model of IL-6-induced JAK1-STAT3 signaling that quantitatively links physiological IL-6 concentrations to the dynamics of IL-6-induced …

0301 basic medicineRuxolitinibruxolitinibPhysiologySystems biologyRegulatorBiologyPharmacology: Biochemistry biophysics & molecular biology [F05] [Life sciences]lcsh:Physiology03 medical and health sciencesMediatoracute phase responsePhysiology (medical)medicineSOCS3primary hepatocytes: Biochimie biophysique & biologie moléculaire [F05] [Sciences du vivant]Original ResearchIL-6lcsh:QP1-981Acute-phase proteinmathematical modelingJAK-STAT signaling pathwayCell biology030104 developmental biologySignal transductionmedicine.drugFrontiers in Physiology
researchProduct

Plant protein phosphorylation monitored by capillary liquid chromatography–element mass spectrometry

2007

Abstract Many essential cellular functions such as growth rate, motility, and metabolic activity are linked to reversible protein phosphorylation, since they are controlled by signaling cascades based mainly on phosphorylation/dephosphorylation events. Quantification of global or site-specific protein phosphorylation is not straightforward with standard proteomic techniques. The coupling of capillary liquid chromatography (μLC) with ICP-MS (inductively coupled plasma-mass spectrometry) is a method which allows a quantitative screening of protein extracts for their phosphorus and sulfur content, and thus provides access to the protein phosphorylation degree. In extension of a recent pilot st…

ProteomicsPhosphataseArabidopsisProtozoan ProteinsBiophysicsChlamydomonas reinhardtiimacromolecular substancesBiologyProteomicsBiochemistryMass SpectrometryDephosphorylationMiceAnimalsProtein phosphorylationPhosphorylationMolecular BiologyCells CulturedPlant ProteinsChromatographyArabidopsis ProteinsPhosphorusCell BiologyPhosphoproteinsbiology.organism_classificationPeptide FragmentsBiochemistryPlant proteinPhosphoproteinPhosphorylationChlamydomonas reinhardtiiSulfurChromatography LiquidBiochemical and Biophysical Research Communications
researchProduct