Assessing the effect of chromium pollution on benthic foraminiferal community: morphological vs. environmental metabarcoding approaches

The purpose of this experimental study is to assess the impact of different concentrations of chromium (Cr) through time on the diversity of cultured benthic foraminifera combining two morphological approaches for the identification of living specimens that are Rose Bengal (RB) staining and CellTracker Green (CTG) labelling as well as environmental DNA and RNA metabarcoding. Seven tanks/aquaria with different concentrations of Cr in water, namely 100 ppt, 1 ppb, 10 ppb, 100 ppb, 1 ppm and 10 ppm plus the control were used. A mesocosm was extracted from each tank at pre-established time (1 week, 2 weeks, 4 weeks 6 weeks and 8 weeks). Morphological analyses produced 6 datasets based on staini…

The response of cultured meiofaunal and benthic foraminiferal communities to lead exposure: Results from mesocosm experiments

2018

Lead (Pb) has been regarded as a very toxic element that poses a serious threat to biota. A mesocosm experiment is performed to assess the influence of Pb on meiofaunal (metazoans within 45-500 µm) and benthic foraminiferal (protozoans) communities. To this end, sediments bearing such communities are incubated in mesocosms, exposed to different levels of Pb in seawater, and monitored for up to eight weeks. Concentrations of Pb below 1 ppm in water do not promote a significant increase of this metal in sediments. Relatively high concentrations of Pb seem to affect meiofaunal and benthic foraminiferal communities by reducing their richness or diversity, and the abundance of the most sensitive…

Environmental RNA outperforms eDNA metabarcoding in assessing impact of marine pollution: A chromium-spiked mesocosm test

2022

Environmental (e)DNA metabarcoding holds great promise for biomonitoring and ecotoxicological applications. However, few studies have compared the performance of eDNA versus eRNA metabarcoding in assessing organismal response to marine pollution, in experimental conditions. Here, we performed a chromium (Cr)-spiked mesocosm experimental test on benthic foraminiferal community to investigate the effects on species diversity by analysing both eDNA and eRNA metabarcoding data across different Cr concentrations in the sediment. Foraminiferal diversity in the eRNA data showed a significant negative correlation with the Cr concentration in the sediment, while a positive response was observed in t…

Distribution of Cr and Pb in artificial sea water and their sorption in marine sediments: an example from experimental mesocosms

2016

The uptake of two heavy metals (chromium and lead) in sediments in experimental mesocosms under exposure to different metal concentrations was evaluated by monitoring their concentrations over time both in seawater and in sediment. Two separate experiments under laboratory-controlled conditions were carried out for the two metals. Sediments were collected from a protected natural area characterized by low anthropic influence and were placed in mesocosms that were housed in aquaria each with seawater at a different metal concentration. At pre-established time intervals, seawater and sediment samples were collected from each mesocosm for chemical analyses. Quantification of chromium and lead …

Comparative high-resolution chemostratigraphy of the Bonarelli Level from the reference Bottaccione section (Umbria-Marche Apennines) and from an equ…

2006

The Bonarelli Level (BL) from the upper Cenomanian portion of the reference Bottaccione section (central Italy) is characterized by the presence of black shales containing high TOC concentrations (up to 17%) and amounts of CaCO3 near to zero. In the absence of carbonate and, consequently, of relative carbon- and oxygen-isotopic data, the elemental geochemistry revealed to be a very useful tool to obtain information about the palaeoclimatic and palaeoceanographic evolution of the Tethys Ocean during the OAE2. Based on several geochemical proxies (Rb, V, Ni, Cr, Si, Ba), the BL is interpreted as a high-productivity event driven by increasingly warm and humid climatic conditions promoting an a…

Integrated stratigraphy of the Oligocene pelagic sequence in the Umbria- Marche basin (north-eastern Apennines, Italy): A potential Global Stratotype…

2008

The Oligocene represents an important time period from a wide range of perspectives and includes signifi cant climatic and eustatic variations. The pelagic succession of the Umbria-Marche Apennines (central Italy) includes a complete and continuous sequence of marly limestones and marls, with volcaniclastic layers that enable us to construct an integrated stratigraphic framework for this time period. We present here a synthesis of detailed biostratigraphic, magnetostratigraphic, and hemostratigraphic studies, along with geochronologic results from several biotite-rich volcaniclastic layers, which provide the means for an accurate and precise radiometric calibration of the Oligocene time sca…

Orbitally modulated black shale deposition in the upper Albian Amadeus Segment (central Italy): a multi-proxy reconstruction

2003

Abstract The upper Albian Amadeus Segment, which falls within Oceanic Anoxic Event 1c, exhibits cyclical alternations of marls/black shales and carbonate-rich beds that record evidence of orbital climate and sedimentary dynamics. A combined micropalaeontological (planktic and benthic foraminifera, and palynomorphs) and geochemical (stable isotopes, clay mineralogy, and major element distribution) investigation allowed recognition of a remarkable influence of continental material within the black shales. Moreover, the palaeoproductivity record is characterised by a non-linear response to the orbital forcing. The micropalaeontological and geochemical record suggests that upper Albian bedded c…

Assessing the effect of mercury pollution on cultured benthic foraminifera community using morphological and eDNA metabarcoding approaches

2018

Mercury (Hg) is a highly toxic element for living organisms and is known to bioaccumulate and biomagnify. Here, we analyze the response of benthic foraminifera communities cultured in mesocosm and exposed to different concentrations of Hg. Standard morphological analyses and environmental DNA metabarcoding show evidence that Hg pollution has detrimental effects on benthic foraminifera. The molecular analysis provides a more complete view of foraminiferal communities including the soft-walled single-chambered monothalamiids and small-sized hard-shelled rotaliids and textulariids than the morphological one. Among these taxa that are typically overlooked in morphological studies we found poten…

High-resolution geochemical and biotic records of the Tethyan 'Bonarelli Level' (OAE2, latest Cenomanian) from the Calabianca-Guidaloca composite sec…

2004

Abstract High-resolution micropalaeontological and chemostratigraphic records for the upper Cenomanian portion of the Calabianca–Guidaloca composite section (NW Sicily) provide new insight into the palaeoclimatic and palaeoceanographic evolution of the Tethys Ocean. The Bonarelli Level equivalent was identified on the basis of lithology and well constrained by calcareous plankton biostratigraphy and radiolarian assemblages, as well as by the δ 13 C curve showing a marked positive excursion (up to 4.7‰). The Bonarelli Level equivalent deposition is characterized by highly eutrophic conditions as testified by radiolarian proliferation. Black shale samples from the Calabianca–Guidaloca composi…