Towards Adoptive Immunotherapy Using High Affinity T Cell Receptors

The hdm2 oncoprotein is frequently overexpressed in a variety of human malignancies, including acute myeloid and lymphoblastic leukemia. Synthetic peptides representative of hdm2 sequences were tested for their binding to A2.1. HLA-A2.1. (/Kb)-Tg mice were immunized with A2.1-binding hdm2 peptides in order to induce A2.1-restricted and hdm2-specific CTL. A2.1-restricted CTL lines obtained from both A2.1 and HuCD8 × A2.1/Kb-Tg mice were able to recognize a synthetic 8-mer peptide representative of a N-terminal hdm2 sequence. These CTL were capable of recognizing and lysing Saos-2 cells transfected with the hdm2 gene as opposed to the parental cell line that lacks any detectable hdm2 expressi…

Influence of the Electrochemical Parameters on the Properties of Electroplated Au-Cu Alloys

Self-Radiolysis of Tritiated Water Stored in Zeolites 4A: Production and Behavior of H 2 and O 2

International audience; Radiolysis of confined water and self-radiolysis of tritiated water give rise to several unanswered questions. To take into account this double specificity, we studied systems of zeolites 4A containing tritiated water at different water loading ratios. Two tritiated waters were synthesized at the volumetric activities of 27 and 60 TBq L −1. For each one, five samples were prepared, differentiated by their water loading ratios, expressed in percentage, close to 4%, 7%, 11%, 14%, and 19%. The study of the radiolysis in those systems revealed a double role of zeolites 4A: first, they increase the decomposition of water. Then they enhance the recombination of the major s…

Phenomenological Model for Gold-Copper Electrodeposition: Application to Thick Coatings

Pure gold-copper alloys are known to be difficult to electrodeposit because of a strong variation in composition after a few microns have been deposited. Commissariat al’Energie Atomique (CEA) stud...

Ultra-low density metallic foams synthesized by contact glow discharge electrolysis (CGDE) for laser experiments★

International audience; The goal of this work is to realize metallic foams synthesized by contact glow discharge electrolysis with specific characteristics. In this paper, we show the results of our studies, consisting in investigating parameters that influence the foams characteristics. Thus, the morphology of metallic foams is examined through scanning electron microscopy (SEM) observations with the acid nature. Moreover, the evolution of the mass and the volume of metallic foams with two experimental parameters (overvoltage and gold concentration) is also investigated. The acid nature affects the foams microscopic structure highlighted by the SEM observations, but for now no valid explan…

Germanium Doped CHxMicroshells for LMJ Targets

AbstractAt the CEA Laser “Megajoule” facility, amorphous hydrogenated carbon (a-C:H or CHx) is the nominal ablator used to achieve inertial confinement fusion experiments. These targets are filled with a fusible mixture of deuterium-tritium in order to perform ignition.Since the achievement of ignition greatly depends on the physical properties of the shell, there must be precise control of thicknesses, doping concentration, and roughness. Experimental devices associated with suitable characterizations are described in this paper. The tolerances and yields for each specification are also presented. Some specifications are largely reached; high-frequency surface roughness due to isolated sur…