Lipid nanocarriers containing esters prodrugs of Flurbiprofen. Preparation, physical-chemical characterization and biological studies.

In this paper, the preparation, chemical-physical, technological and in vitro characterization of nanostructured lipid carriers (NLC) carrying R-flurbiprofen ester prodrugs, were analyzed for a potential pharmaceutical application. R-flurbiprofen was chosen as a model drug because it has been found to play an effective role in counteracting secretases involved in neurodegenerative diseases, although it does not cross the Blood Brain Barrier (BBB). In this study, two R-flurbiprofen ester prodrugs (ethyl and hexyl) were successfully synthesized and entrapped into non-pegylated and pegylated NLC. The obtained systems showed average diameters in the colloidal size range, negative zeta potential…

Preventive Impact of Long-Term Ingestion of Chestnut Honey on Glucose Disorders and Neurodegeneration in Obese Mice

The purpose of the present study was to evaluate the impact of long-term honey ingestion on metabolic disorders and neurodegeneration in mice fed a high-fat diet (HFD). Three groups of mice were fed with a standard diet (STD), HFD or HFD supplemented with honey (HFD-H) for 16 weeks. Biochemical, histological, Western blotting, RT-PCR and Profiler PCR array were performed to assess metabolic parameters, peripheral and central insulin resistance and neurodegeneration. Daily honey intake prevented the HFD-induced glucose dysmetabolism. In fact, it reduced plasma fasting glucose, insulin and leptin concentrations and increased adiponectin levels. It improved glucose tolerance, insulin sensitivi…

Toxic effects induced by recombinant Ab 42 Peptide

SOLID LIPID NANOPARTICLES CONTAINING AN ANTIOXIDANT COMPOUND IN ALZHEIMER’S DISEASE

RAPD (Random Amplified Polymorphic DNA) profiles distinguish Paracentrotus lividus populations living in a stressing environment (Amvrakikos Gulf, Greece)

Random amplified polymorphic DNA (RAPD) analysis was performed to assess genetic markers of Paracentrotus lividus populations living in stressing environment in the Amvrakikos Gulf (Western Greece, Ionian Sea) where two populations distinguishable in body size, smaller than the open sea ones, were detected. The UPGMA dendrogram, constructed from pairwise . st values among population nuclear DNA markers, revealed that the small and medium-sized populations living inside the Amvrakikos presented a lower polymorphism, and form a cluster that shows the genetic distance with normal-sized populations (Ionian and Tyrrhenian Seas) living in open sea. AMOVA analysis indicated a genetic distance amon…

SOLID LIPID NANOPARTICLES (SLNs) CONTAINING FERULIC ACID AGAINST OXIDATIVE STRESS IN NEURODEGENERATIVE DISEASES

Toxicity of recombinant β-amyloid prefibrillar oligomers on morphogenesis of Sea Urchin Paracentrotus lividus.

Effetti del peptide Abeta(1-42) sullo sviluppo embrionale del paracentrotus lividus

In vitro reactivity of peripheral blood mononuclear cells from subjects with different genetic background to amyloid b (Ab42) peptide: implication for cancer an immunotherapy.