0000000000075573

AUTHOR

Gustav Waschatko

showing 4 related works from this author

Photo-DHEA--a functional photoreactive dehydroepiandrosterone (DHEA) analog.

2011

Abstract The steroid hormone dehydroepiandrosterone (DHEA) has beneficial effects on vascular function, survival of neurons, and fatty acid metabolism. However, a specific receptor for DHEA has not been identified to date. Here, we describe the synthesis of a photoreactive DHEA derivative (Photo-DHEA). In Photo-DHEA, typical characteristics of DHEA are conserved: (i) a “planar” tetracyclic ring system with a Δ 5 double bond, (ii) a 3β-hydroxyl group, and (iii) a keto group at C17. In cell-based assays, Photo-DHEA showed the same properties as DHEA. We conclude that Photo-DHEA is suitable for radioiodination to yield a tool for the identification of the elusive DHEA receptor.

endocrine systemmedicine.medical_specialtyReceptors SteroidDouble bondPhotochemistrymedicine.medical_treatmentClinical BiochemistryDehydroepiandrosteroneBiochemistrychemistry.chemical_compoundEndocrinologyInternal medicinepolycyclic compoundsmedicineHumansskin and connective tissue diseasesReceptorMolecular BiologyG protein-coupled receptorPharmacologychemistry.chemical_classificationFatty acid metabolismPhotoaffinity labelingOrganic ChemistryDHEA receptorDehydroepiandrosteroneSteroid hormoneEndocrinologychemistryMolecular Probeshuman activitieshormones hormone substitutes and hormone antagonistsSteroids
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Fast and direct analysis of oxidation levels of oil-in-water emulsions using ATR-FTIR.

2019

International audience; Oxidation of omega-3 fatty acids is a major limitation on its enrichment in food and beverages. An efficient and simple method to monitor lipid oxidation in complex systems is essential to limit lipid oxidation during formulation and processing. Fish oil-in-water emulsions (20% v/v) were exposed to iron or free radical initiated oxidation. Conjugated dienes (CDs) were rapidly measured using a previously developed fat extraction method. Fourier transform infrared (FTIR) spectroscopy has been used to directly record chemical changes occurring during oxidation. Variations were noticed in different spectral regions despite the presence of broad water bands near 3400 and …

IronAnalytical chemistryInfrared spectroscopyFish oil01 natural sciencesAnalytical ChemistryChemometrics0404 agricultural biotechnologyFish OilsLipid oxidation[SDV.IDA]Life Sciences [q-bio]/Food engineeringPartial least squares regressionFatty Acids Omega-3Spectroscopy Fourier Transform InfraredChemometricsFourier transform infrared spectroscopyLeast-Squares AnalysisSpectroscopyInfrared spectroscopyChemistryOil-in-water emulsions010401 analytical chemistryExtraction (chemistry)Water04 agricultural and veterinary sciencesGeneral Medicine[SDV.IDA] Life Sciences [q-bio]/Food engineering040401 food scienceLipid oxidation0104 chemical sciencesRadical initiatorRadical initiatorEmulsions[CHIM.OTHE]Chemical Sciences/OtherOxidation-ReductionFood ScienceFood chemistry
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DHEA-Bodipy–a functional fluorescent DHEA analog for live cell imaging

2009

International audience; The androgen dehydroepiandrosterone (DHEA) has been reported to protect neuronal cells against dysfunction and apoptosis. Several signaling pathways involved in these effects have been described but little is known about the intracellular trafficking of DHEA. We describe design, synthesis and characterization of DHEA-Bodipy, a novel fluorescent DHEA analog. DHEA-Bodipy proved to be a functional DHEA derivative: DHEA-Bodipy (i) induced estrogen receptor α-mediated gene activation, (ii) protected PC12 rat pheochromocytoma cells against serum deprivation-induced apoptosis, and (iii) induced stress fibers and focal adhesion contacts in SH-SY5Y human neuroblastoma cells. …

Boron CompoundsDHEA-Bodipyendocrine systemDehydroepiandrosteroneEstrogen receptorApoptosisBiologyPC12 CellsBiochemistryfluorescence microscopyCell membranegenomicNeuroblastoma03 medical and health sciences0302 clinical medicineEndocrinologynon-genomicGenes ReporterLive cell imagingtraffickingmedicinepolycyclic compoundsAnimalsHumansskin and connective tissue diseasesMolecular BiologyFluorescent Dyes030304 developmental biology0303 health sciencesMolecular StructureCell MembraneEstrogen Receptor alphaBiological TransportDehydroepiandrosteroneRats3. Good healthCell biologylive cell imagingmedicine.anatomical_structureMicroscopy FluorescenceApoptosisSignal transductionEstrogen receptor alphahuman activities030217 neurology & neurosurgeryIntracellularhormones hormone substitutes and hormone antagonists
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The role of intact oleosin for stabilization and function of oleosomes.

2013

Lipid storage in plants is achieved among all plant species by formation of oleosomes, enclosing oil (triacylglycerides) in small subcellular droplets. Seeds are rich in this pre-emulsified oil to provide a sufficient energy reservoir for growing. The triacylglyceride core of the oleosomes is surrounded by a phospholipid monolayer containing densely packed proteins called oleosins. They are anchored in the triacylglycerides core with a hydrophobic domain, while the hydrophilic termini remain on the surface. These specialized proteins are expressed during seed development and maturation. Particularly, they play a major role in the stabilization and function of oleosomes. To better understand…

Enzymatic digestionChemistryAirCircular DichroismPhospholipidWaterHydrogen-Ion ConcentrationLipid storageSurfaces Coatings and Filmschemistry.chemical_compoundBiochemistrySpectroscopy Fourier Transform InfraredMaterials ChemistryPlant speciesWater chemistryHelianthusPlant OilsEmulsionsSoybeansPhysical and Theoretical ChemistryOleosinFunction (biology)Plant ProteinsThe journal of physical chemistry. B
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