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RESEARCH PRODUCT
DHEA-Bodipy–a functional fluorescent DHEA analog for live cell imaging
Gustav WaschatkoNorbert BertramAnita BoppDieter LütjohannChristoph HönnscheidtKatja Gehrig-burgerSusanne Lemckesubject
Boron CompoundsDHEA-Bodipyendocrine systemDehydroepiandrosteroneEstrogen receptorApoptosisBiologyPC12 CellsBiochemistryfluorescence microscopyCell membranegenomicNeuroblastoma03 medical and health sciences0302 clinical medicineEndocrinologynon-genomicGenes ReporterLive cell imagingtraffickingmedicinepolycyclic compoundsAnimalsHumansskin and connective tissue diseasesMolecular BiologyFluorescent Dyes030304 developmental biology0303 health sciencesMolecular StructureCell MembraneEstrogen Receptor alphaBiological TransportDehydroepiandrosteroneRats3. Good healthCell biologylive cell imagingmedicine.anatomical_structureMicroscopy FluorescenceApoptosisSignal transductionEstrogen receptor alphahuman activities030217 neurology & neurosurgeryIntracellularhormones hormone substitutes and hormone antagonistsdescription
International audience; The androgen dehydroepiandrosterone (DHEA) has been reported to protect neuronal cells against dysfunction and apoptosis. Several signaling pathways involved in these effects have been described but little is known about the intracellular trafficking of DHEA. We describe design, synthesis and characterization of DHEA-Bodipy, a novel fluorescent DHEA analog. DHEA-Bodipy proved to be a functional DHEA derivative: DHEA-Bodipy (i) induced estrogen receptor α-mediated gene activation, (ii) protected PC12 rat pheochromocytoma cells against serum deprivation-induced apoptosis, and (iii) induced stress fibers and focal adhesion contacts in SH-SY5Y human neuroblastoma cells. DHEA-Bodipy bound rapidly and specifically to plasma membranes of living PC12 cells. We analyzed metabolism and trafficking of DHEA-Bodipy in human neuroblastoma cells. DHEA-Bodipy is the first functional fluorescent DHEA derivative suitable for live cell imaging of intracellular DHEA transport and localization.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-10-24 |