0000000000083121

AUTHOR

Jintong Zhao

showing 2 related works from this author

Negative pressure cavitation extraction and antioxidant activity of genistein and genistin from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]

2010

Abstract A new method—negative pressure cavitation extraction (NPCE) was proposed and investigated for the extraction of the main isoflavonoids, namely genistein and genistin from pigeon pea roots. The effects of extraction time and particle size on the extraction yields were firstly optimized, then a central composite design (CCD) combined with response surface methodology (RSM) was used to study the effects of negative pressure, ethanol concentration and liquid/solid ratio on the extraction yields. The maximum extraction yields of genistein and genistin reached 0.418 and 0.398 mg/g, respectively, under the optimal conditions: extraction time 45 min, particle size 50 mesh, negative pressur…

ChromatographyCentral composite designbiologyDPPHExtraction (chemistry)GenisteinFiltration and Separationbiology.organism_classificationAnalytical Chemistrychemistry.chemical_compoundCajanuschemistryGenistinParticle sizeResponse surface methodologySeparation and Purification Technology
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Antioxidant properties, superoxide dismutase and glutathione reductase activities in HepG2 cells with a fungal endophyte producing apigenin from pige…

2012

Abstract A fungal endophyte MD89 with obvious antioxidant activities was isolated from pigeon pea and identified as Chaetomium globosum by ITS sequence. Different fractions from MD89 culture were compared and evaluated by total phenol (TP) content, total flavonoid (TFL) content, DPPH radical scavenging, reducing power and lipid peroxidation assays, respectively. Results showed that EtOAc extracts had high content of TP and TFL, and strong antioxidant activities (IC 50 value was 6.87, 15.19, 16.78 μg/mL, respectively). Furthermore, the EtOAc extracts were analyzed by LC–MS/MS and a good antioxidant compound, apigenin, was found. The activities of superoxide dismutase (SOD) and glutathione re…

chemistry.chemical_classificationAntioxidantbiologyDPPHmedicine.medical_treatmentGlutathione reductaseFlavonoidbiology.organism_classificationLipid peroxidationSuperoxide dismutasechemistry.chemical_compoundCajanuschemistryBiochemistryApigeninmedicinebiology.proteinFood scienceFood ScienceFood Research International
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