0000000000083388

AUTHOR

Paola Mazzetti

showing 1 related works from this author

Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune compete…

2019

Background Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict posttransplant immune-related complications, and eventually to customize immunosuppression. Methods In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of the TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE assay. Results In the validation study, the TTV R-GENE showed good performances in precision and reproducibility, and sensitivity as low as 12 TTV DNA copies/mL, like previously reported for the in-house rtPCR. The Bl…

Concordancedigital droplet PCR; methods comparison; real-time PCR; torquetenovirusViremiaTTVBiologyReal-Time Polymerase Chain ReactionComparative evaluation03 medical and health sciencesTTV; digital droplet PCR; methods comparison; real-time PCR0302 clinical medicineImmune systemVirologymedicineHumans030212 general & internal medicineViremiaDigital droplet pcrTorque teno virusSurrogate endpointReproducibility of Resultsmedicine.diseaseVirologyDNA Virus InfectionsQuantitative measuretorquetenovirusInfectious DiseasesReal-time polymerase chain reactionCase-Control StudiesDNA Viral030211 gastroenterology & hepatologyreal-time PCRImmunocompetencedigital droplet PCRBiomarkersmethods comparison
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