0000000000083580
AUTHOR
J. J. De Monserrat
showing 3 related works from this author
Computer assisted morphometric analysis of ram sperm heads: Evaluation of different fixative techniques
1998
The recent development of automated systems for morphometric sperm head analysis has provided a series of objective parameters which have facilitated the standardization of morphological semen evaluation. This current work attempts to establish the optimum fixing conditions for the morphometric characterization of ram spermatozoa. Ejaculates were obtained from 5 Merino rams used for periodic collection of semen and were diluted at 1:50 with TEST medium. Air-dried smears were fixed either in ethanol-ether (1:1), 50% methanol, 2% glutaraldehyde or SUZA fixative, in which case the smear was pretreated with chloramine. The samples were then stained with commercial kit Hemacolor. Once the prepar…
Regionalization of epididymal duct and epithelium in rats and mice by automatic computer-aided morphometric analysis.
2005
Aim: To establish a rat and mouse epididymal map based on the use of the Epiquatre automatic software for histologic image analysis. Methods: Epididymides from five adult rats and five adult mice were fixed in alcoholic Bouin’s fixative and embedded in paraffin. Serial longitudinal sections through the medial aspect of the organ were cut at 10 µm and stained with hematoxylin and eosin. As determined from major connective tissue septa, nine subdivisions of the rat epididymis and seven for the mouse were determined, consisting of five sub-regions in the caput (rat and mouse), one (mouse) or three (rat) in the corpus and one in the cauda (rat and mouse). Using the Epiquatre software, several t…
Use of the Sperm-Class Analyser® for objective assessment of human sperm morphology
2003
The Sperm-Class Analyser was validated for assessing morphometric parameters of the head and midpiece of unwashed and washed human ejaculated spermatozoa from volunteers providing a wide range of semen quality. A higher proportion of sperm could be assessed (86% fresh semen and 75% washed sperm) if Hemacolor staining was used rather than DiffQuik (80 and 73%) or Papanicolaou (78 and 68%). Different stains employed different fixatives and the area, length, width and perimeter of the sperm head was significantly larger for washed sperm stained by Hemacolor and DiffQuik. Acrosomal area ranged from 48 to 51% of the sperm head area and this percentage was larger for washed sperm stained with Dif…