0000000000084234

AUTHOR

Soon Thye Lim

showing 3 related works from this author

T-Cell Lymphoma Clonality by Copy Number Variation Analysis of T-Cell Receptor Genes

2021

Simple Summary T-cells defend the human body from pathogenic invasion via specific recognition by T-cell receptors (TCRs). The TCR genes undergo recombination (rearrangement) in a myriad of possible ways to generate different TCRs that can recognize a wide diversity of foreign antigens. However, in patients with T-cell lymphoma (TCL), a particular T-cell becomes malignant and proliferates, resulting in a population of genetically identical cells with same TCR rearrangement pattern. To help diagnose patients with TCL, a polymerase chain reaction (PCR)-based assay is currently used to determine if neoplastic cells in patient samples are of T-cell origin and bear identical (monoclonal) TCR rea…

0301 basic medicineclone (Java method)Cancer ResearchclonalityBiologylcsh:RC254-282Article03 medical and health sciences0302 clinical medicinemedicineT-cell lymphomaT-cell receptorCopy-number variationcopy number variation analysisGeneWhole genome sequencingwhole genome sequencingT-cell receptorlcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensmedicine.diseaseMolecular biology030104 developmental biologyOncology030220 oncology & carcinogenesisT-Cell Receptor GeneMonoclonalT-cell lymphomaClonality Copy number variation analysis T-cell lymphoma T-cell receptor Whole genome sequencingCancers
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Abstract PO-35: Prognostic significance of MYC, BCL2, and BCL6 colocalization at single-cell resolution in DLBCL

2020

Abstract MYC, BCL2, and BCL6 are commonly used markers for immunohistochemistry of Diffuse large B-cell lymphomas (DLBCL). Coexpression of MYC and BCL2 in particular constitutes a subgroup of “double expressor lymphomas” (DEL) with a distinct poor clinical outcome. However, it is not known if MYC and BCL2/BCL6 coexpression occurs in the same cell or in different cells within the tumor, as traditional immunohistochemistry (IHC) is limited by the number of markers that can be simultaneously assessed within formalin-fixed, paraffin-embedded (FFPE) samples. We set out to discover the clinical significance of MYC, BCL2, and BCL6 colocalization at single-cell resolution using multiplexed quantita…

CellGerminal centerColocalizationGeneral MedicineBiologyBCL6Continuous variablemedicine.anatomical_structureimmune system diseaseshemic and lymphatic diseasesTonsilCancer researchmedicineImmunohistochemistryClinical significanceBlood Cancer Discovery
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Patterns of oncogene co-expression at single cell resolution in cancer influence survival

2020

AbstractBackgroundCancers often overexpress multiple clinically relevant oncogenes. However, it is not known if multiple oncogenes within a cancer combine uniquely in specific cellular sub-populations to influence clinical outcome. We studied this phenomenon using the prognostically relevant oncogenes MYC, BCL2 and BCL6 in Diffuse Large B-Cell Lymphoma (DLBCL).MethodsQuantitative multispectral imaging simultaneously measured oncogene co-expression at single-cell resolution in reactive lymphoid tissue (n=12) and four independent cohorts (n=409) of DLBCL. Mathematically derived co-expression phenotypes were evaluated in DLBCLs with immunohistochemistry (n=316) and eight DLBCL cohorts with gen…

Quantitative immunohistochemistryCellBiologyBCL6medicine.diseasemedicine.anatomical_structureimmune system diseaseshemic and lymphatic diseasesGene expressionCo localisationmedicineCancer researchImmunohistochemistryneoplasmsDiffuse large B-cell lymphomaPatient stratification
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