0000000000086292
AUTHOR
S.a. Merzouk
showing 9 related works from this author
Time course of changes in serum oxidant/antioxidant status in overfed obese rats and their offspring.
2009
The aim of the present study was to determine the time course of changes in oxidant/antioxidant status, as well as serum glucose, insulin, leptin and lipid levels, liver adipose tissue and muscle lipid and protein contents, in cafeteria-diet-fed dams during gestation and lactation, and in their offspring throughout adulthood. Food intake was also evaluated. The cafeteria diet induced a significant increase in maternal body and relative adipose tissue weights, daily energy intake, and plasma glucose, insulin, leptin and lipid levels at parturition (day 0) and at the end of lactation (day 21). Plasma total antioxidant status [ORAC (oxygen radical absorbance capacity)], erythrocyte catalase an…
2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine stimule la prolifération in vitro des lymphocytes humains et la sécrétion des interleukines
2008
Resume But de l’etude Il a ete demontre que les aziridines possedent une activite immunotropique marquee. Dans ce travail, les effets in vitro d’une aziridine nouvellement synthetisee (2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine) sont determines sur la proliferation des lymphocytes humains stimules par les agents mitogenes, et sur la secretion des interleukines 2 et 6 (IL-2, IL-6). Materiel et methodes Les lymphocytes du sang peripherique sont isoles par centrifugation differentielle dans un gradient de Ficoll-Paque. Ils sont mis en culture en presence d’agents mitogenes (concanavaline A, lipopolysaccharide) et de differentes concentrations de l’aziridine testee. La determination de …
Effect of Dietaryn-3Polyunsaturated Fatty Acids on Oxidant/Antioxidant Status in Macrosomic Offspring of Diabetic Rats
2014
The aim of this work was to determine the effect of dietaryn-3PUFA on oxidant/antioxidant status,in vitrovery low and low density lipoprotein (VLDL-LDL), and VLDL-LDL-fatty acid composition in macrosomic pups of diabetic mothers. We hypothesized thatn-3PUFA would improve oxidative stress in macrosomia. Diabetes was induced in female Wistar rats fed with the ISIO diet (control) or with the EPAX diet (enriched inn-3PUFAs), by streptozotocin. The macrosomic pups were killed at birth (day 0) and at adulthood (day 90). Lipid parameters and VLDL-LDL-fatty acid composition were investigated. The oxidant/antioxidant status was determined by measuring plasma oxygen radical absorbance capacity (ORAC)…
N-3 fatty acids modulate antioxidant status in diabetic rats and their macrosomic offspring.
2006
We investigated the role of dietary n-3 polyunsaturated fatty acids (n-3 PUFA) in the modulation of total antioxidant status in streptozotocin (STZ)-induced diabetic rats and their macrosomic offspring. Female wistar rats, fed on control diet or n-3 PUFA diet, were rendered diabetic by administration of five mild doses of STZ on day 5 and were killed on days 12 and 21 of gestation. The macrosomic (MAC) pups were killed at the age of 60 and 90 days. Lipid peroxidation was measured as the concentrations of plasma thiobarbituric acid reactive substances (TBARS), and the total antioxidant status was determined by measuring (i) plasma oxygen radical absorbance capacity (ORAC), (ii) plasma vitami…
Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: a comparison of older with young men.
2011
In this study, plasma lipids, lipoproteins and markers of oxidant/antioxidant status were investigated in young (n = 45) and older (n = 40) obese men and compared to those in young (n = 65) and older (n = 55) normal weight controls. The purpose was to determine whether obesity exacerbates or not lipid, lipoprotein abnormalities and oxidative stress in older men. Our findings showed that all obese patients had increased plasma triglyceride, cholesterol, LDL-cholesterol, -triglyceride and HDL-triglyceride levels concentrations compared to controls (P < 0.01). However, the younger obese men had relatively larger and accentuated changes in plasma lipids and lipoproteins than the older patients.…
Oxidative stress and maternal obesity: Feto-placental unit interaction
2013
Abstract Objective To determine oxidative stress markers in maternal obesity during pregnancy and to evaluate feto-placental unit interaction, especially predictors of fetal metabolic alterations. Patients and methods 40 obese pregnant women (prepregnancy BMI > 30 kg/m²) were compared to 50 control pregnant women. Maternal, cord blood and placenta samples were collected at delivery. Biochemical parameters (total cholesterol and triglycerides) and oxidative stress markers (malondialdehyde, carbonyl proteins, superoxide anion expressed as reduced Nitroblue Tetrazolium, nitric oxide expressed as nitrite, reduced glutathione, catalase, superoxide dismutase) were assayed by biochemical methods. …
Effects of Mancozeb and Metribuzin on in vitro proliferative responses and oxidative stress of human and rat spleen lymphocytes stimulated by mitogens
2011
Pesticides have been shown to possess marked immunotropic activity. The aim of this work was to study the in vitro effects of different concentrations (1-100 µM) of Mancozeb (fungicide) and Metribuzin (herbicide), on the proliferative responses of human and rat spleen lymphocytes stimulated by concanavalin A (ConA, mitogen), the Th1- (IL-2, INFγ) and Th2- (IL-4) cytokine secretion and on the intracellular oxidative status. The results showed that Mancozeb significantly reduced ConA lymphocyte proliferation in a dose-dependent manner in both humans and rats. It also decreased IL-2, INFγ and IL-4 secretion with a a shift away to Th1 phenotype. Metribuzin at low concentrations (1-10 µM) result…
Chronic low level metribuzin exposure induces metabolic alterations in rats
2013
Abstract In this work we evaluated the in vivo effects of chronic metribuzin exposure at doses that mimic human exposure through diet. Male and female rats were fed a potato diet containing metribuzin at low doses (D1, 1.3 mg/kg or D2, 13 mg/kg) for 3 months. Plasma biochemical parameters (glucose, lipid, urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST)), as well as lipid and protein contents, markers of oxidative stress in different organs (liver, adipose tissue, muscle, intestine) were determined. Our results showed that exposure to metribuzin induced a significant reduction in body weight, food intake and adverse alterations in biochemical parameters suc…
Altered adipose tissue metabolism in offspring of dietary obese rat dams.
2011
To investigate further the mechanisms of developmental programming, we analysed the effects of maternal overnutrition and of postnatal high-fat feeding on adipose tissue metabolism in the offspring. Postnatal changes in serum adiponectin, leptin and TAG [triacylglycerol (triglyceride)] levels, adipose tissue TAGs, fatty acids and enzyme activities were determined in offspring of cafeteria-diet-fed dams during gestation and lactation, weaned on to standard chow or on to cafeteria diet. Obese rats showed higher adiposity (+35% to 85%) as well as a significant increase in serum glucose, insulin, leptin, adiponectin and TAG levels (P&lt;0.01) and adipose tissue LPL (lipoprotein lipase) and …