0000000000087951
AUTHOR
Päivi Eskelinen
Suppression of Saprolegnia infections in rainbow trout (Oncorhynchus mykiss) eggs using protective bacteria and ultraviolet irradiation of the hatchery water
Since formalin is widely used in prevention of Sapro- legnia infections in salmonid fish hatcheries, there is a need for more environmentally safe treatment methods. Therefore, we screened 360 bacterial iso- lates against their ability to antagonize the growth of Saprolegnia parasitica hyphae in vitro, and best strains were selected according to their antagonistic proper- ties and colonization capability on rainbow trout egg surface. Protective bacterial cultures of Pseudomonas sp. M162, Pseudomonas sp. M174 and Janthinobacte- rium M169 were tested for prevention of Saprolegnia sp. infections during incubation trials of rainbow trout (Oncorhynchus mykiss )e ggs with UV irradiated (400 mWs c…
Efficacy of clove oil and ethanol against Saprolegnia sp. and usability as antifungal agents during incubation of rainbow trout Oncorhynchus mykiss (Walbaum) eggs
Inhibitory concentrations of clove oil and ethanol against growth of Saprolegnia sp. hyphae were screened by a modification of the hemp (Cannabis sativa L.) seed MicroPlate (HeMP) method and their usability as antifungal agents during incubation of rainbow trout Oncorhynchus mykiss eggs was tested. In vitro experiment showed that in continuous static exposure, clove oil at 100 mg L−1 significantly inhibited the growth of Saprolegnia, whereas in bath exposures, clove oil at 500 mg L−1 had no significant effect at any exposure time tested (15, 60 and 240 min), but clove oil at 10 000 mg L−1 significantly inhibited growth at all exposure times. Clove oil and ethanol treatments had no visible e…
Tutkimus ja seuranta
Kalavarojen käyttö ja hoito pysyvät kestävällä pohjalla, kun päätökset perustetaan mahdollisimman luotettavaan ja ajantasaiseen tietoon. Tietoa tarvitaan toiminnan kaikissa vaiheissa, mutta erityisesti silloin, kun asetetaan tavoitetilaa ja osatavoitteita sekä myöhemmin, kun on aika arvioida toimenpiteiden tuloksellisuutta. Tässä luvussa esitellään alueelliseen käyttöön soveltuvia tiedonhankintamenetelmiä sekä annetaan ohjeita aineistojen keruuseen ja tulosten tulkintaan. nonPeerReviewed
Effects of soybean meal based diet on growth performance, gut histopathology and intestinal microbiota of juvenile rainbow trout (Oncorhynchus mykiss)
Abstract Effects of soybean meal (SBM) based diet on growth performance, histology of the intestinal epithelium and on the gut microbiota of juvenile rainbow trout (Oncorhynchus mykiss) were investigated on two trials lasting for 8 weeks (Trial I) and 18 weeks (Trial II). The microbiological characterization was done both with conventional plating techniques, biochemical profiling and length heterogeneity analysis of PCR amplified 16S rDNA (LH-PCR). Typical histological changes were found after 18 weeks of SBM feeding (Trial II). Mean height of simple foldings were significantly higher within the group fed with fish meal (FM) based diet. These changes were not, however, reflected in the app…