0000000000115759

AUTHOR

Hassan S. Khalid

showing 6 related works from this author

Cytotoxicity of the bisphenolic honokiol from Magnolia officinalis against multiple drug-resistant tumor cells as determined by pharmacogenomics and …

2014

A main problem in oncology is the development of drug-resistance. Some plant-derived lignans are established in cancer therapy, e.g. the semisynthetic epipodophyllotoxins etoposide and teniposide. Their activity is, unfortunately, hampered by the ATP-binding cassette (ABC) efflux transporter, P-glycoprotein. Here, we investigated the bisphenolic honokiol derived from Magnolia officinalis. P-glycoprotein-overexpressing CEM/ADR5000 cells were not cross-resistant to honokiol, but MDA-MB-231 BRCP cells transfected with another ABC-transporter, BCRP, revealed 3-fold resistance. Further drug resistance mechanisms analyzed study was the tumor suppressor TP53 and the epidermal growth factor recepto…

HonokiolATP Binding Cassette Transporter Subfamily BPharmaceutical ScienceBiologyPharmacologyLignanschemistry.chemical_compoundGefitinibCell Line TumorDrug DiscoverymedicineATP Binding Cassette Transporter Subfamily G Member 2HumansEpidermal growth factor receptorCytotoxicityPI3K/AKT/mTOR pathwayOligonucleotide Array Sequence AnalysisPharmacologyBiphenyl CompoundsTransfectionbiology.organism_classificationAntineoplastic Agents PhytogenicDrug Resistance MultipleNeoplasm ProteinsErbB ReceptorsMolecular Docking SimulationMagnolia officinalisComplementary and alternative medicinechemistryDrug Resistance NeoplasmMagnoliaPharmacogeneticsbiology.proteinMolecular MedicineATP-Binding Cassette TransportersErlotinibTumor Suppressor Protein p53Transcriptomemedicine.drugSignal TransductionPhytomedicine : international journal of phytotherapy and phytopharmacology
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Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.

2015

Abstract Background Ziziphus spina-christi (L.) Desf. (Christ's Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects. Nowadays, Z. spina-christi, is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation. Pharmacological research undertaken to date suggests that it possesses anti-inflammatory, hypoglycemic, hypotensive and anti-microbial e…

0301 basic medicinemedicine.drug_classLeupeptinsIn silicoHerbal MedicineEgypt AncientAnti-Inflammatory AgentsPharmaceutical SciencePlant RootsAnti-inflammatory03 medical and health sciencesCell Line TumorDrug DiscoveryMedicineGallocatechinBioassayHumansElectrophoretic mobility shift assayHistory AncientPharmacologyZiziphus spina-christiInflammationPlants MedicinalbiologyTraditional medicinePlant Stemsbusiness.industryPlant ExtractsTranscription Factor RelAZiziphusZiziphusbiology.organism_classificationMolecular Docking Simulation030104 developmental biologyComplementary and alternative medicineDocking (molecular)SeedsMolecular MedicinebusinessPhytomedicine : international journal of phytotherapy and phytopharmacology
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Activity of the dietary flavonoid, apigenin, against multidrug-resistant tumor cells as determined by pharmacogenomics and molecular docking

2015

Apigenin is a common dietary flavonoid with considerable cytotoxic activity in vitro and in vivo. Despite many mechanistic studies, less is known about resistance factors hampering apigenin's activity. We investigated the ATP-binding cassette (ABC) transporters BCRP/ABCG2, P-glycoprotein/ABCB1 and its close relative ABCB5. Multidrug-resistant cells overexpressing these ABC transporters were not cross-resistant toward apigenin. Moreover, apigenin inhibited not only P-glycoprotein but also BCRP by increasing cellular uptake of doxorubicin and synergistic inhibition of cell viability in combination with doxorubicin or docetaxel in multidrug-resistant cells. To perform in silico molecular docki…

Cancer ResearchAbcg2Protein ConformationEndocrinology Diabetes and MetabolismClinical BiochemistryATP-binding cassette transporterPharmacologyBiochemistryMicechemistry.chemical_compoundTranscriptional regulationCluster AnalysisImmunology and AllergyApigeninNutrition and DieteticsbiologyABCB5Drug Resistance MultipleNeoplasm ProteinsMolecular Docking SimulationOncologyBiochemistryApigeninMolecular Medicinemedicine.drugIn silicoImmunologyInhibitory Concentration 50Cell Line TumormedicineAnimalsHumansDoxorubicinATP Binding Cassette Transporter Subfamily B Member 1RNA MessengerViability assayMolecular BiologyPharmacologyComputational BiologyPolyphenolsTransporterIn vitroHEK293 CellschemistryDoxorubicinDrug Resistance NeoplasmPharmacogeneticsPoster Presentationbiology.proteinATP-Binding Cassette TransportersThe Journal of Nutritional Biochemistry
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The lignan, (-)-sesamin reveals cytotoxicity toward cancer cells: pharmacogenomic determination of genes associated with sensitivity or resistance.

2013

(-)-Sesamin is a lignan present in sesam oil and a number of medicinal plants. It exerts various pharmacological effects, such as prevention of hyperlipidemia, hypertension, and carcinogenesis. Moreover, (-)-sesamin has chemopreventive and anticancer activity in vitro and in vivo. Multidrug resistance (MDR) of tumors leads to fatal treatment outcome in many patients and novel drugs able to kill multidrug-resistant cells are urgently needed. P-glycoprotein (MDR1/ABCB1) is the best known ATP-binding cassette (ABC) drug transporter mediating MDR. ABCB5 is a close relative to ABCB1, which also mediates MDR. We found that the mRNA expressions of ABCB1 and ABCB5 were not related to the 50% inhibi…

ATP Binding Cassette Transporter Subfamily BPharmaceutical ScienceATP-binding cassette transporterDioxolesBiologyPharmacologymedicine.disease_causeLignanschemistry.chemical_compoundSesaminCell Line TumorNeoplasmsDrug DiscoverymedicineCluster AnalysisHumansATP Binding Cassette Transporter Subfamily B Member 1GenePharmacologyPlant ExtractsGene Expression ProfilingABCB5Multiple drug resistanceMitochondrial respiratory chainHEK293 CellsComplementary and alternative medicinechemistryDrug Resistance NeoplasmCancer cellMolecular MedicineDrug Screening Assays AntitumorCarcinogenesisPhytotherapyPhytomedicine : international journal of phytotherapy and phytopharmacology
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Cytotoxicity of cucurbitacin E from Citrullus colocynthis against multidrug-resistant cancer cells

2019

Abstract Background Cucurbitacin E (CuE) is an oxygenated tetracyclic triterpenoid isolated from the fruits of Citrullus colocynthis (L.) Schrad. Purpose This study outlines CuE's cytotoxic activity against drug-resistant tumor cell lines. Three members of ABC transporters superfamily, P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and ABCB5 were investigated, whose overexpression in tumors is tightly linked to multidrug resistance. Further factors of drug resistance studied were the tumor suppressor TP53 and the epidermal growth factor receptor (EGFR). Methods Cytotoxicity assays (resazurin assays) were used to investigate the activity of Citrullus colocynthis and CuE towar…

Abcg2Drug ResistancePharmaceutical ScienceATP-binding cassette transporterMicroarraySubfamily Gchemistry.chemical_compoundGene Knockout Techniques0302 clinical medicineEpidermal growth factorPhytogenicDrug DiscoveryATP Binding Cassette Transporter Subfamily G Member 2Cancer0303 health sciencesTumorLeukemiabiologyChemistryABCB5TransfectionCell cycleNeoplasm ProteinsGene Expression Regulation NeoplasticErbB ReceptorsMolecular Docking SimulationSubfamily B030220 oncology & carcinogenesisMolecular MedicineCitrullus colocynthiMember 2Member 1ATP Binding Cassette Transporter Subfamily BATP Binding Cassette TransporterAntineoplastic AgentsCell Line03 medical and health sciencesCell Line TumorHumansATP Binding Cassette Transporter Subfamily B Member 1030304 developmental biologyCucurbitacin EPharmacologyNeoplasticTraditional herbal medicineCancer; Citrullus colocynthis; Drug resistance; Microarray; Traditional herbal medicine; ATP Binding Cassette Transporter Subfamily B; ATP Binding Cassette Transporter Subfamily B Member 1; ATP Binding Cassette Transporter Subfamily G Member 2; Antineoplastic Agents Phytogenic; Cell Line Tumor; Citrullus colocynthis; Doxorubicin; Drug Resistance Neoplasm; ErbB Receptors; Gene Expression Regulation Neoplastic; Gene Knockout Techniques; Humans; Leukemia; Molecular Docking Simulation; Neoplasm Proteins; Triterpenes; Tumor Suppressor Protein p53Antineoplastic Agents PhytogenicTriterpenesComplementary and alternative medicineGene Expression RegulationDrug Resistance NeoplasmDoxorubicinCancer cellbiology.proteinCancer researchNeoplasmCitrullus colocynthisTumor Suppressor Protein p53
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Pharmacogenomic and molecular docking studies on the cytotoxicity of the natural steroid wortmannin against multidrug-resistant tumor cells

2014

Wortmannin is a cytotoxic compound derived from the endophytic fungi Fusarium oxysporum, Penicillium wortmannii and Penicillium funiculosum that occurs in many plants, including medicinal herbs. The rationale to develop novel anticancer drugs is the frequent development of tumor resistance to the existing antineoplasic agents. Therefore, it is mandatory to analyze resistance mechanisms of novel drug candidates such as wortmannin as well to bring effective drugs into the clinic that have the potential to bypass or overcome resistance to established drugs and to substantially increase life span of cancer patients. In the present project, we found that P-glycoprotein-overexpressing tumor cells…

DrugATP Binding Cassette Transporter Subfamily BClass I Phosphatidylinositol 3-Kinasesmedia_common.quotation_subjectPharmaceutical ScienceAntineoplastic AgentsATP-binding cassette transporterDrug resistancePharmacologyBiologyWortmanninPhosphatidylinositol 3-Kinaseschemistry.chemical_compoundCell Line TumorDrug DiscoveryCluster AnalysisHumansCytotoxicityProtein kinase BPI3K/AKT/mTOR pathwayOligonucleotide Array Sequence Analysismedia_commonPharmacologyDrug Resistance MultipleAndrostadienesMolecular Docking SimulationMultiple drug resistanceComplementary and alternative medicinechemistryDrug Resistance NeoplasmPharmacogeneticsMolecular MedicineWortmanninSignal TransductionPhytomedicine
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