0000000000116494

AUTHOR

Marilena Anastasio

showing 3 related works from this author

Use of nodulation pattern, stress tolerance, nodC gene amplification, RAPD-PCR and RFLP-16S rDNA analysis to discriminate genotypes of Rhizobium legu…

2005

Twenty-seven new Rhizobium isolates were obtained from root nodules of wild and crop legumes belonging to the genera Vicia, Lathyrus and Pisum from different agroecological areas in central and southern Italy. A polyphasic approach including phenotypic and genotypic techniques was used to study their diversity and their relationships with other biovars and species of rhizobia. Analysis of symbiotic properties and stress tolerance tests revealed that wild isolates, showed a wide spectrum of nodulation and a marked variation in stress tolerance compared with reference strains tested in this study. All rhizobial isolates (except for the isolate CG4 from Galega officinalis) were presumptively i…

DNA BacterialRoot noduleGenotypeStress toleranceBiologymedicine.disease_causeN-AcetylglucosaminyltransferasesApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalPolymerase Chain ReactionMediterranean areaRhizobium leguminosarumRhizobiaBacterial ProteinsRhizobium leguninosarumNodC geneStress toleranceWild legumesStrains diversityMediterranean areaSymbiotic characteristicsRNA Ribosomal 16SmedicineSymbiosisEcology Evolution Behavior and SystematicsGeneticsPrincipal Component AnalysisRhizobium leguminosarumfood and beveragesFabaceaeNucleic acid amplification techniqueNodC geneHydrogen-Ion ConcentrationRhizobium leguninosarum16S ribosomal RNAbiology.organism_classificationStrains diversitySymbiotic characteristicsRAPDBacterial Typing TechniquesRandom Amplified Polymorphic DNA TechniqueRhizobiumWild legumeRestriction fragment length polymorphismNucleic Acid Amplification TechniquesPolymorphism Restriction Fragment LengthSettore AGR/16 - Microbiologia Agraria
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Heterotrophic microorganisms in deteriorated medieval wall paintings in southern Italian churches

2008

The Campania region in southern Italy is noted for its large number of churches that harbour invaluable frescoes, dated from the beginnings of the 4th up to the 13th century. The wall paintings represent an integral part of the monuments, and their deterioration constitutes a potentially significant loss for the world's cultural heritage. Heterotrophic microorganisms such as bacteria and mould can grow on the surface of paintings that contain a wide range of organic and inorganic constituents, and provide different ecological. niches that are exploited by a large variety of microbial. species. We isolated and identified the heterotrophic microorganisms found in the biodegraded medieval wall…

DNA BacterialIdentificationMedieval wall paintingMicroorganismMolecular Sequence DataBacillus cereusHeterotrophic microorganismDNA RibosomalMicrobiologyPaenibacillusGenusRNA Ribosomal 16SBotanyWall paintingDeteriorationDNA FungalPhylogenyBacillus (shape)BacteriabiologyActinomyceteBacillus pumilusFungiHeterotrophic ProcessesAlternariabiology.organism_classification16S ribosomal RNAHistory MedievalhumanitiesBiodeteriorationItalyPaintingsSettore AGR/16 - Microbiologia AgrariaMicrobiological Research
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Technological and molecular diversity of Lactobacillus plantarum strains isolated from naturally fermented sourdoughs.

2004

Thirty Lactobacillus (L.) plantarum strains, isolated from sourdough, were identified by biochemical tests as well as 16S rDNA sequencing and differentiated on the basis of technological properties, such as amylase, protease, phytase and antirope activities. These properties were shown to be widely differing among the strains, indicating a significant technological diversity. Genetic differentiation was achieved by restriction endonuclease analysis-pulsed field gel electrophoresis (REA-PFGE) that allowed the L. plantarum strains to be divided into 10 different genomic groups. Moreover, 32 different starters were employed in dough making experiments; each starter consisted of a single strain…

Fermentation starterMolecular Sequence DataApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalStarterLactobacillusRNA Ribosomal 16SFood scienceAmylaseEcology Evolution Behavior and SystematicsLeavening agentLactobacillus plantarum – starter cultures – sourdough – molecular differentiation – technological properties – dough makingbiologyfood and beveragesGenetic VariationBreadSequence Analysis DNAbiology.organism_classificationYeastLactobacillus plantarumstarter culturessourdoughmolecular differentiationtechnological propertiesdough makingLactobacillusFermentationbiology.proteinbacteriaFermentationLactobacillus plantarumSettore AGR/16 - Microbiologia AgrariaSystematic and applied microbiology
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