0000000000116650
AUTHOR
Kateryna Filippovych
Computational Modeling of Protein Dynamics in Eukaryotic Cells
Proteins have important functions inside the cell, traveling diffusively or being actively transported to various cellular sites where their activity is needed. Protein motion in the cellular environment is therefore an important topic to understand. However, the cell provides a very complex environment for that motion, which poses problems especially for any modeling effort designed to interpret experimentally observed features. So as to gain a realistic picture of protein dynamics inside the cell, we have recently introduced advanced numerical methods for describing that dynamics [1]. The starting point is an accurate numerical duplicate of the cell determined by LSCM, which can be used a…
Computational modeling of protein diffusion in the cell nucleus using the lattice-Boltzmann method
The number of fluorescence applications and methods of analysis is growing in the biological and related sciences. The aim of this work was to accurately determine the diffusion coefficient in nucleus, diffusion in the cytoplasm and nucleo-cytoplasmic translocation should be considered as well. Fluorescence recovery after photobleaching (FRAP) experiments were used in order to study protein diffusion. A numerical model for FRAP was used to determine the diffusion properties of proteins. The present method removed the difficulties of the conventional analysis, could produce, interesting results for protein diffusion in the cell.