0000000000117093
AUTHOR
Jean-christophe Lata
Agricultural management affects the response of soil bacterial community structure and respiration to water-stress
International audience; Soil microorganisms are responsible for organic matter decomposition processes that regulate soil carbon storage and mineralisation to CO2. Climate change is predicted to increase the frequency of drought events, with uncertain consequences for soil microbial communities. In this study we tested the hypothesis that agricultural management used to enhance soil carbon stocks would increase the stability of microbial community structure and activity in response to water-stress. Soil was sampled from a long-term field trial with three soil carbon management systems and was used in a laboratory study of the effect of a dry wet cycle on organic C mineralisation and microbi…
Characterization of Bacterial and Fungal Soil Communities by Automated Ribosomal Intergenic Spacer Analysis Fingerprints: Biological and Methodological Variability
ABSTRACT Automated rRNA intergenic spacer analysis (ARISA) was used to characterise bacterial (B-ARISA) and fungal (F-ARISA) communities from different soil types. The 16S-23S intergenic spacer region from the bacterial rRNA operon was amplified from total soil community DNA for B-ARISA. Similarly, the two internal transcribed spacers and the 5.8S rRNA gene (ITS1-5.8S-ITS2) from the fungal rRNA operon were amplified from total soil community DNA for F-ARISA. Universal fluorescence-labeled primers were used for the PCRs, and fragments of between 200 and 1,200 bp were resolved on denaturing polyacrylamide gels by use of an automated sequencer with laser detection. Methodological (DNA extracti…
Increase inabovegroundfreshlitterquantityover-stimulatessoil respiration inatemperatedeciduousforest
In the context of climate change, the amount of carbon allocated to soil, particularly fresh litter, is predicted to increase with terrestrial ecosystem productivity, and may alter soil carbon storage capacities. In this study we performed a 1-year litter-manipulation experiment to examine how soil CO2 efflux was altered by the amount of fresh litter. Three treatments were applied: litter exclusion (E), control (C, natural amount: 486 g m −2 ) and litter addition (A, twice the natural amount: 972 g m −2
Seasonal dynamics of the bacterial community in forest soils under different quantities of leaf litter
International audience; Soil microbial communities play an important role in soil carbon functioning, particularly in forest ecosystems. Their variation in response to climate change may affect soil carbon processes, highlighting the importance of understanding how environmental factors affect microbial communities. This study aimed to determine to what extent an increase in the quantity of fresh litter may affect heterotrophic mineralization of organic carbon and bacterial community structure in soil and litter. A litter manipulation experiment was performed in situ in a temperate deciduous forest. Three treatments of fresh litter inputs were considered: litter exclusion, natural condition…