0000000000117371
AUTHOR
Julie Foulon
Poplar biomass production at phytomanagement sites is significantly enhanced by mycorrhizal inoculation
Abstract Afforestation of trace element (TE)-contaminated soil, notably with fast growing trees producing large biomass has been demonstrated to be an attractive option for their bioremediation. Mycorrhizal fungi can form symbiotic associations with plants, contributing to TE tolerance and participating actively in bioremediation processes. We studied the effects of mycorrhizal inoculation on the growth of two poplar clones (Skado and I214), to large-scale. Two TE-contaminated sites of ca . 1 ha (Pierrelaye and Fresnes-sur-Escaut, France) were planted with 2200 trees, and were either inoculated with a mixed commercial inoculum or not-inoculated and allometric parameters were determined afte…
Environmental metabarcoding reveals contrasting microbial communities at two poplar phytomanagement sites
The aim of the present study is to deepen the current understanding of the microbial communities at two poplar phytomanagement sites to reveal the environmental factors that drive the abundance, diversity and composition of microbial communities. A soil analysis revealed that the two soils displayed contrasting physico-chemical characteristics, with significant lower pH and higher Cd, Zn and Mn CaCl2-extractable fractions at Leforest site, compared with Pierrelaye site. The fungal and bacterial community profiles in the poplar roots and soils were assessed through Illumina MiSeq sequencing. Diversity indices and β-diversity measures illustrated that the root microbial communities were well …
Diversity and complexity of microbial communities from a chlor-alkali tailings dump
Abstract Revegetation of the tailings dumps produced by various industrial activities is necessary to prevent dust storms and erosion and represents a great challenge for ecological restoration. Little is known about the microbial colonisation and community structure of revegetated tailings following site exploitation. Here, we report the sequencing of 16S rRNA and internal transcribed spacer (ITS) fungal RNA gene amplicons from chlor-alkali residue and from an adjacent undisturbed soil to define the composition and assembly of the rhizosphere microbial communities. After quality filtering, a total of 72,373 and 89,929 bacterial sequences and 122,618 and 111,209 fungal sequences remained fo…
Plant growth promotion, metabolite production and metal tolerance of dark septate endophytes isolated from metal-polluted poplar phytomanagement sites
Numerous studies address the distribution and the diversity of dark septate endophytes (DSEs) in the literature, but little is known about their ecological role and their effect on host plants, especially in metal-polluted soils. Seven DSE strains belonging to Cadophora, Leptodontidium, Phialophora and Phialocephala were isolated from roots of poplar trees from metal-polluted sites. All strains developed on a wide range of carbohydrates, including cell-wall-related compounds. The strains evenly colonized birch, eucalyptus and ryegrass roots in re-synthesis experiments. Root and shoot growth promotion was observed and was both plant and strain dependent. Two Phialophora and Leptodontidium st…
Impact of poplar-based phytomanagement on soil properties and microbial communities in a metal-contaminated site
Despite a long history of use in phytomanagement strategies, the impacts of poplar trees on the structure and function of microbial communities that live in the soil remain largely unknown. The current study combined fungal and bacterial community analyses from different management regimes using Illumina-based sequencing with soil analysis. The poplar phytomanagement regimes led to a significant increase in soil fertility and a decreased bioavailability of Zn and Cd, in concert with changes in the microbial communities. The most notable changes in the relative abundance of taxa and operational taxonomic units unsurprisingly indicated that root and soil constitute distinct ecological microbi…