0000000000119520
AUTHOR
R. Tuan
PLLA Scaffold with Gradient pore size in microphysiological tissue system bioreactor for Osteochondral regeneration
Cartilage and bone tissues in the joints are intimately linked and form the osteochondral unit. A better understanding of both disease and regenerative processes of bone and cartilage requires the study of both tissues together, as part of the osteochondral unit to account for their mutual interactions. However, the production of scaffolds for osteochondral tissue regeneration is a challenging task, since scaffolds must mimic the differents morphologies of cartilage and bone. Thermally Induced Phase Separation (TIPS) is one of the most adaptable techniques to produce porous scaffold for Tissue Engineering applications. A wide range of morphologies in terms of both pore size and distribution…
A Poly-L-Lactide scaffold with continuous gradient pore size for osteochondral regeneration validated in a microphysiological tissue system bioreactor
A microphysiological tissue system (MPS) bioreactor has been developed to replicate in vitro the in vivo OC physiological conditions. The MPS allows separate control of the chondral and osseous environment while permitting communication between chondrocytes and osteoblasts across the OC junction, similar to the conditions of OC tissue in vivo. We have used here our MPS system to validate the TIPS -generated pore-gradient PLLA scaffold.
A Continuous Pore Size Gradient PLLA Scaffold For Osteochondral Regeneration
Osteochondral (OC) scaffold-based regenerative approaches in the joint are challenging since the scaffold must provide mechanical strength while also mimicking the local cartilage and bone microenvironments. Thermally Induced Phase Separation (TIPS) can produce scaffolds with a wide range of pore size morphologies/distributions. Here, we produced by TIPS a poly-L-lactide (PLL A) scaffold with a continuous pore size gradient along the sample thickness, from ~70μm diameter on one side to ~200 μm diameter on the opposite surface.