CHROMIUM | Properties and Determination

Antioxidant capacity of infant fruit beverages: influence of storage and in vitro gastrointestinal digestion

Reyes.Barbera@uv.es; M.J.Lagarda@uv.es; Rosaura.Farre@uv.es The total antioxidant capacity of three beverages based on fruit juice, milk and cereals, intended for infants and young children up to 3 years of age was evaluated by two methods Trolox Equivalent Antioxidant Capacity and Oxygen Radical Absorption Capacity. Results: According to the total antioxidant values obtained by both methods, the beverages can be ranked as follows: grape-orange-banana > peach-apple > pineapple-banana. Ascorbic acid was the main contributor (60%) to the total antioxidant capacity, while the contribution of skimmed milk was less than 1.2%. After one month of storage at -20 ºC, significant losses (p < 0.05) in…

Abstracts of papers and posters safe handling of medicines

A Quantitative Estimate of Ascorbic and Isoascorbic Acid by High Performance Liquid Chromatography: Application to Citric Juices

Abstract The present report deals with the optimization and verification of a simple HPLC isocratic method useful for determining ascorbic and isoascorbic acids in standard solutions and fruit juices. Sample preparation is minimal and docs not require derivatization. The method uses an octadecyl reversed phase, a mobile phase which contains cetyltrimethyl ammonium bromide 0.05M as the ionic pair and potassium dihydrogen phosphate as a buffer, at pH=4.5, and 4-hydro-xyacetanilide as internal standard. The proposed method makes it possible to quantify ascorbic and isoascorbic acid in less than 13 minutes, with precision (C. V. 3.5%) and accuracy (recover' of 98%).

Abstracts of papers and posters advanced activities in pharmaceutical care 24th European Symposium on Clinical Pharmacy