0000000000123232

AUTHOR

Paola Ronzi

showing 4 related works from this author

Stable changes in CD4+ T lymphocyte miRNA expression after exposure to HIV-1

2012

Abstract MicroRNAs (miRNAs) inhibit HIV-1 expression by either modulating host innate immunity or by directly interfering with viral mRNAs. We evaluated the expression of 377 miRNAs in CD4+ T cells from HIV-1 élite long-term nonprogressors (éLTNPs), naive patients, and multiply exposed uninfected (MEU) patients, and we observed that the éLTNP patients clustered with naive patients, whereas all MEU subjects grouped together. The discriminatory power of miRNAs showed that 21 miRNAs significantly differentiated éLTNP from MEU patients and 23 miRNAs distinguished naive from MEU patients, whereas only 1 miRNA (miR-155) discriminated éLTNP from naive patients. We proposed that miRNA expression ma…

AdultCD4-Positive T-LymphocytesMaleTime FactorsImmunologyHIV InfectionsHIV Envelope Protein gp120BiologyBiochemistryImmune systemmultiply exposed uninfectedmicroRNAHumansDroshamiRNAInnate immune systemélite long-term nonprogressorsGene Expression ProfilingCell BiologyHematologyT lymphocyteMiddle AgedViral LoadMicroarray AnalysisHIV-1; miRNA; CD4+ T cells; élite long-term nonprogressors; multiply exposed uninfected.CD4+ T cellsIn vitroMicroRNAsGene Expression RegulationCase-Control StudiesImmunologyHIV-1biology.proteinFemaleEx vivoDicerBlood
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Changes in CD4+ cells’ miRNA expression following exposure to HIV-1

2010

Background: Micro RNAs (miRNAs) inhibit HIV-1 expression by either modulating host innate immunity or by directly interfering with viral mRNAs. Here, we investigated the miRNA profile that discriminates different classes of HIV-1-infected patients from multiple-exposed uninfected individuals. Methods: The expression levels of 377 miRNAs were selectively analysed in CD4+ cells isolated from whole blood of HIV-1 elite LTNP (eLTNP), naive, and multiply exposed uninfected individuals (MEUs). MiRNA extraction was performed by the mir Vana™ miRNA Isolation Kit (Ambion), and their expression was subsequently examined by real-time PCR-based arrays. The expression of miRNAs was also determined in pr…

Innate immune systembiologybusiness.industryHuman immunodeficiency virus (HIV)Public Health Environmental and Occupational Healthmedicine.disease_causemedicine.diseaseIn vitroImmune systemInfectious DiseasesAcquired immunodeficiency syndrome (AIDS)microRNAImmunologymedicinebiology.proteinOral PresentationbusinessDroshaDicerJournal of the International AIDS Society
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Antibody Complementarity-Determining Regions (CDRs) Can Display Differential Antimicrobial, Antiviral and Antitumor Activities

2008

9 p. Background: Complementarity-determining regions (CDRs) are immunoglobulin (Ig) hypervariable domains that determine specific antibody (Ab) binding. We have shown that synthetic CDR-related peptides and many decapeptides spanning the variable region of a recombinant yeast killer toxin-like antiidiotypic Ab are candidacidal in vitro. An alanine-substituted decapeptide from the variable region of this Ab displayed increased cytotoxicity in vitro and/or therapeutic effects in vivo against various bacteria, fungi, protozoa and viruses. The possibility that isolated CDRs, represented by short synthetic peptides, may display antimicrobial, antiviral and antitumor activities irrespective of Ab…

Antifungal AgentsBIOCHEMISTRY AND MOLECULAR BIOLOGYMolecular Sequence DataImmunologylcsh:MedicineAntineoplastic AgentsMicrobial Sensitivity TestsComplementarity determining regionBiologyAntiviral AgentsOncology/Skin CancersAntibodiesMiceMicrobiology/Applied MicrobiologyAntigenBiochemistry/Protein ChemistryInfectious Diseases/Fungal InfectionsIn vivoCell Line TumorCandida albicansInfectious Diseases/Viral InfectionsAnimalsHumansAmino Acid Sequencelcsh:SciencePeptide sequenceMultidisciplinaryMEDICINElcsh:RAntimicrobialComplementarity Determining RegionsVirologyIn vitroOncologyBiochemistryViral replicationAGRICULTURAL AND BIOLOGICAL SCIENCESVirology/Immunodeficiency VirusesHIV-1biology.proteinlcsh:QAntibodyResearch ArticlePLoS ONE
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A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents

2016

AbstractA phosphorylated peptide, named K40H, derived from the constant region of IgMs was detected in human serum by liquid chromatography coupled to high-resolution mass spectrometry. Synthetic K40H proved to exert a potent in vitro activity against fungal pathogens, and to inhibit HIV-1 replication in vitro and ex vivo. It also showed a therapeutic effect against an experimental infection by Candida albicans in the invertebrate model Galleria mellonella. K40H represents the proof of concept of the innate role that naturally occurring antibody fragments may exert against infectious agents, shedding a new light upon the posthumous role of antibodies and opening a new scenario on the multif…

0301 basic medicineMicrobial Sensitivity TestsVirus ReplicationArticleMass SpectrometryMicrobiology03 medical and health sciencesAnti-Infective AgentsCandida albicansHumansPhosphorylationCandida albicansInfectivityMultidisciplinaryInnate immune system030102 biochemistry & molecular biologybiologybiology.organism_classificationVirologyPeptide FragmentsIn vitroImmunoglobulin Fc FragmentsGalleria mellonella030104 developmental biologyImmunoglobulin MHumoral immunityHIV-1biology.proteinAntibodyEx vivoChromatography LiquidScientific Reports
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