0000000000133206

AUTHOR

G. Galluzzo

showing 2 related works from this author

Analysis of T-Lymphocyte Subsets After Phytohemagglutinin Stimulation in Normal and Type 1 Diabetic Mothers and Their Infants

1992

Our aim was to investigate the immunological status of diabetic pregnancy, which is an overlap of diabetic immunity abnormalities and the immunological modifications normally occurring during pregnancy. METHOD: We studied lymphocyte subpopulations and lymphokine production, after 96 h of phytohemagglutinin (PHA) stimulation, from normal and Type I diabetic pregnant women at delivery time and from the respective cord blood. RESULTS: Peripheral blood mononuclear cells (PBMC) from both normal and Type I diabetic mothers showed an increase in CD8+ and a decrease in CD4+ cells compared to the respective cord blood mononuclear cells (CBMC). Moreover, Type I PBMC showed a lower number of “activate…

Adultmedicine.medical_specialtyCellular immunityCD3ImmunologyPregnancy in DiabeticsLymphocyte ActivationPeripheral blood mononuclear cellInterferon-gammaAntigens CDPregnancyT-Lymphocyte SubsetsInternal medicinemedicineHumansImmunology and AllergyIL-2 receptorPhytohemagglutininsCells CulturedImmunity CellularbiologyInterleukin-6Tumor Necrosis Factor-alphabusiness.industryLymphokineObstetrics and GynecologyReceptors Interleukin-2HLA-DR AntigensT lymphocyteFetal BloodFlow CytometryDiabetes Mellitus Type 1EndocrinologyReproductive MedicineCord bloodImmunologybiology.proteinInterleukin-2FemalebusinessCD8Interleukin-1American Journal of Reproductive Immunology
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Study of T-cell activation in Type I diabetic patients and pre-Type I diabetic subjects by cytometric analysis: Antigen expression defectin vitro

1993

In Type I diabetes the observation of a decreased release of interleukin-2 (IL-2) and soluble IL-2 receptors by means of stimulated lymphocytes in vitro indicates that a primary immunoregulatory defect may be involved. To confirm this hypothesis we investigated the T-cell activation trend, evaluating the surface expression of IL-2 receptor (CD25), transferrin (CD71), HLA class II (DR), and CD69 phenotypes after in vitro stimulation with phytohemagglutinin (PHA; 1 and 10 micrograms/ml) and concanavalin A (12.5 micrograms/ml) in six newly diagnosed Type I diabetics and six islet cell- and insulin autoantibody-positive first-degree relatives. As controls were studied six long-standing Type I d…

AdultAntigens Differentiation T-LymphocyteCD4-Positive T-LymphocytesMaleInterleukin 2medicine.medical_specialtyTime FactorsAdolescentCD3 ComplexCD8 AntigensT-Lymphocytesmedicine.medical_treatmentT cellImmunologyTransferrin receptorBiologyLymphocyte ActivationAntigenAntigens CDInternal medicineDiabetes mellitusReceptors TransferrinmedicineHumansImmunology and AllergyLectins C-TypeIL-2 receptorPhytohemagglutininsReceptorInsulinReceptors Interleukin-2HLA-DR Antigensmedicine.diseaseAntigens Differentiation B-LymphocyteKineticsDiabetes Mellitus Type 1Endocrinologymedicine.anatomical_structureInterleukin-2Femalemedicine.drugJournal of Clinical Immunology
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