0000000000133853
AUTHOR
Julia Llorca
DNA Amplification Fingerprinting for Subtyping Neisseria gonorrhoeae Strains
Background and Objectives DNA amplification fingerprinting is used in most epidemiologic studies as a substitute for conventional typing methods. DNA amplification fingerprinting and conventional typing methods were compared in this epidemiologic study of Neisseria gonorrhoeae. Goal of This Study To differentiate 70 Neisseria gonorrhoeae isolates from untreated patients with urogenital gonococcal infection. Study Design Gonococcal strains were characterized by auxo-typing, serotyping, plasmid profile, antibiotic sensitivity, and DNA amplification fingerprinting. The method of unweighted pair-group average linkage was used for cluster analysis. Discriminatory power was calculated applying Si…
Phenobarbital Induction of UDP-glucosyltransferase Activity in Drosophila melanogaster Meigen
The inducibility of UDP-glucosyltransferase activities towards the exogenous substrates 1-naphthol and 2-naphthol and the endogenous metabolite xanthurenic acid was demonstrated in Drosophila melanogaster Meigen larvae and adults using phenobarbital as an inducer. In adults, a 3.5-fold increase of glucosyltransferase activity toward xanthurenic acid and a 2.0-fold increase of the activity toward exogenous substrates (1-naphthol and 2-naphthol) was found. In larvae, maximum induction of all three UDP-glucosyltransferase activities (2.5-fold and 1.5-fold increase of the activity toward the exogenous and endogenous substrates, respectively) was achieved when insects, reared on solid medium, we…
Separation by FPLC chromatofocusing of UDP-glucosyltransferases from three developmental stages of Drosophila melanogaster.
Variation of UDP-glucosyltransferase activity, during Drosophila melanogaster development, was analyzed. The endogenous metabolite xanthurenic acid and the xenobiotic compounds 1-naphthol and 2-naphthol were used as substrates. Developmentally regulated differences were observed for the three substrates, suggesting the presence of UDP-glucosyltransferase isoenzymes. This was further confirmed by FPLC chromatofocusing on a Mono P column: seven peaks of UDP-glucosyltransferase activity (pHs: ≥6.3, 5.8, 5.5, 4.9, 4.5, 4.2, ≤4.0) with either single or overlapping substrate specificity were detected. A single xanthurenic acid:UDP-glucosyltransferase activity (pl 5.8) was found throughout develop…