0000000000133904

AUTHOR

J Schwing

showing 3 related works from this author

Costimulatory signalling potential of murine MHC class II‐positive T‐clone cells

1996

Activated human and rat T cells as well as mouse T-cell clones have been reported to synthesize and express major histocompatibility complex (MHC) class II molecules. However, the capacity of class II+ antigen (Ag) presenting T cells to induce proliferation of Ag-specific cloned T cells has been controversial. We analysed whether the failure of some T-cell clones to proliferate in response to Ag presented by class II+ T cells is because of a lack of costimulatory cytokine production by the antigen-presenting cells (APC). As a model system the mouse class II+ cloned BI/O4.1 T cells were used as APC in order to activate the T cell clone KIII5. This T-helper 1 (Th1) type, GAT (synthetic copoly…

ImmunologyAntigen presentationCD1Antigen-Presenting CellsPolymerase Chain ReactionCell LineMiceInterleukin 21T-Lymphocyte SubsetsAnimalsImmunology and AllergyCytotoxic T cellIL-2 receptorAntigen-presenting cellMice Inbred C3HMHC class IICD40biologyHistocompatibility Antigens Class IIReceptors Interleukin-2Th1 CellsInterleukin-12Molecular biologyMice Inbred C57BLbiology.proteinInterleukin-2Cell DivisionSpleenSignal TransductionResearch ArticleImmunology
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Modulation of proliferation and lymphokine secretion of murine CD4+ T cells and cloned Th1 cells by proteins of the extracellular matrix.

1997

In this study we investigated the co-stimulatory signaling capacity of diverse proteins of the extracellular matrix (ECM) for murine resting CD4+ T cells and Th1 clone cells, activated by immobilized anti-CD3 mAb. ECM proteins used in various concentrations had no effect on IL-2 production or proliferation of highly purified CD4+ T cell populations. When the preparation of CD4+ T cells contained contaminating accessory cells, IL-2 secretion and proliferation was enhanced in the presence of co-immobilized collagens or fibronectin. However, the level of proliferation attainable by added irradiated splenocytes was not reached. Using Th1 cell clone M4, enhanced production of IL-2 in the presenc…

CD4-Positive T-LymphocytesT cellImmunologyLymphocyte ActivationExtracellular matrixInterleukin 21MicemedicineImmunology and AllergyCytotoxic T cellAnimalsSecretionAntigen-presenting cellExtracellular Matrix ProteinsLymphokinesMice Inbred BALB CMice Inbred C3HbiologyChemistryIntegrin beta1LymphokineReceptors Interleukin-2General MedicineTh1 CellsMolecular biologyCell biologyClone CellsFibronectinMice Inbred C57BLmedicine.anatomical_structurebiology.proteinInternational immunology
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Involvement of NO in contact hypersensitivity.

1998

The NO synthases (NOS) generate NO from L-arginine. High concentrations of NO have been shown to be responsible for tissue injury and cell death, while low concentrations of NO induce vasodilatation and other signaling effects. We have investigated the involvement of NO in contact hypersensitivity (CHS) reactions. CHS induced by treatment of BALB/c mice with the contact allergen 2,4-dinitrofluorobenzene (DNFB) was significantly reduced by the NOS inhibitor N-methyl-L-arginine (L-NMA), but not by the stereoisomer D-NMA, as shown by reduced ear swelling responses and evaluation of ear tissue sections. The CHS response was also reduced by aminoguanidine, which is known to preferentially inhibi…

Programmed cell deathLangerhans cellArginineInjections IntradermalT-LymphocytesImmunologyNitric Oxide Synthase Type IIBiologyArginineDermatitis ContactNitric OxideGuanidineschemistry.chemical_compoundMicemedicineImmunology and AllergyAnimalsRNA MessengerEnzyme InhibitorsSkinMice Inbred BALB Cintegumentary systemEpidermis (botany)Histocompatibility Antigens Class IIGeneral MedicineAllergensMolecular biologyPimagedineNitric oxide synthasemedicine.anatomical_structurechemistryLangerhans Cellsbiology.proteinDinitrofluorobenzeneSignal transductionNitric Oxide SynthaseKeratinocyteHaptensInternational immunology
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