0000000000136254
AUTHOR
W. Förster
Establishment and Characterization of Two Merkel Cell Tumor Cultures
Two Merkel cell tumor cultures (MC-MA1, MC-MA2) have been established from metastases of typical Merkel cell tumors. The mestastases in vivo were characterized by co-expression of cytokeratins 8, 18, 19, 20 and neurofilaments, presence of intermediate filament whirls, expression of synaptophysin, neuron-specific enolase, and chromogranin A, rare and weak immunostaining for plakoglobin but absence of cadherins and desmoplakins. Both cultures grow, using supplemented RPMI medium on human irradiated fibroblast feeder layers, as loosely arranged floating small aggregates. Their karyotypes are mostly hyperdiploid. The mean doubling times were about 84 h in the first 8 months and later increased.…
Über die Möglichkeit des Vorkommens doppelgipfliger Häufigkeitsverteilungen bei homogenem Material
Our experiments suggest that a M-shaped curve of frequency distribution does not need to be always the result of non-homogeneous materiali.