0000000000136283

AUTHOR

M.l. Lospalluti

showing 2 related works from this author

Fecal assays detect hypersensitivity to cow's milk protein and gluten in adults with irritable bowel syndrome.

2011

Background & Aims Some patients with irritable bowel syndrome (IBS)-like symptoms suffer from food hypersensitivity (FH); their symptoms improve when they are placed on elimination diets. No assays identify patients with FH with satisfactory levels of sensitivity. We determined the frequency of FH among patients with symptoms of IBS and the ability of fecal assays for tryptase, eosinophil cationic protein (ECP), or calprotectin to diagnose FH. Methods The study included 160 patients with IBS, 40 patients with other gastrointestinal diseases, and 50 healthy individuals (controls). At the start of the study, patients completed a symptom severity questionnaire, fecal samples were assayed, and …

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaFecal Calprotectin.AdolescentGlutensTryptaseWheat HypersensitivityGastroenterologyIrritable Bowel SyndromePlacebosFecesYoung Adultfluids and secretionsDouble-Blind MethodInternal medicineElimination dietmedicineHumansIrritable bowel syndromeFeceschemistry.chemical_classificationEosinophil cationic proteinHepatologybiologybusiness.industryOral food challengeEosinophil Cationic ProteinGastroenterologyMiddle Agedmedicine.diseaseGlutenDietchemistryGluten SensitivityImmunologybiology.proteinFemaleTryptasesCalprotectinCow’s Milk Protein HypersensitivityMilk HypersensitivitybusinessLeukocyte L1 Antigen ComplexCow’s Milk Protein Hypersensitivity; Gluten Sensitivity; Fecal Tryptase; Fecal Eosinophil Cationic Protein; Fecal Calprotectin.Fecal TryptaseFecal Eosinophil Cationic ProteinClinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
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A Cytologic Assay for Diagnosis of Food Hypersensitivity in Patients With Irritable Bowel Syndrome

2010

BACKGROUND & AIMS: A percentage of patients with symptoms of irritable bowel syndrome (IBS) suffer from food hypersensitivity (FH) and improve on a food-elimination diet. No assays have satisfactory levels of sensitivity for identifying patients with FH. We evaluated the efficacy of an in vitro basophil activation assay in the diagnosis of FH in IBS-like patients. METHODS: Blood samples were collected from 120 consecutive patients diagnosed with IBS according to Rome II criteria. We analyzed in vitro activation of basophils by food allergens (based on levels of CD63 expression), as well as total and food-specific immunoglobulin (Ig)E levels in serum. Effects of elimination diets and dou…

AdultMalefood hypersensitivitySettore MED/09 - Medicina InternaAdolescentCytologic assay; food hypersensitivity; irritable bowel diseaseCytological Techniquesirritable bowel diseasePlatelet Membrane GlycoproteinsImmunoglobulin ESensitivity and SpecificityIrritable Bowel SyndromeYoung AdultAntigenAntigens CDElimination dietmedicineAnimalsHumansYoung adultCells CulturedIrritable bowel syndromeCytologic assayHepatologybiologyCD63Tetraspanin 30business.industryGastroenterologyAllergensImmunoglobulin EMiddle Agedmedicine.diseaseBasophilsBasophil activationImmunologybiology.proteinFemaleAntibodybusinessClinical Gastroenterology and Hepatology
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