0000000000138468
AUTHOR
Elodie Maitrepierre
showing 8 related works from this author
Strategy for recombinant expression of functional N-terminal domain of human T1R3 taste receptor produced in Escherichia Coli
2009
International audience
Expression hétérologue, repliement in vitro et caractérisation biophysique du domaine N-terminal de la sous-unité T1R3 du récepteur humain au goût su…
2010
The sweet taste receptor is a heterodimer composed of two subunits called T1R2 and T1R3. Each subunit belongs to the class C of G protein-coupled receptors and is constituted by a large extracellular N-terminal domain (NTD) linked to the transmembrane domain by a cysteine-rich region. It has been shown that T1R2 and T1R3 NTDs are both able to bind natural sugars and sucralose with distinct affinities and undergo ligand-dependent conformational change (Nie et al., Curr Biol, 2005). However, the binding properties of T1R3 NTD and the relative contribution of the two subunits to the heterodimeric receptor function remained largely unknown. To characterize the binding properties of each subunit…
Les molécules odorantes, sapides et trigéminales
2012
National audience
Flavor receptors
2011
National audience
Purification, biophysical characterization and first crystallization trials of the ligand-binding domain of the human T1R3 sweet taste receptor
2013
International audience
Expression of functional N-terminal domain of human T1R2 taste receptor
2011
International audience
Expression and characterization of ligand-binding domain of T1R1 taste receptor
2010
International audience; Umami is the typical taste induced by monosodium glutamate,which is thought to be detected by a heterodimeric G-proteincoupled receptors, T1R1 and T1R3. The most unique feature ofumami taste is its potentiation by purine nucleotidemonophosphates (IMP, GMP), which also elicit umami taste bytheir own. Zhang et al. (Proc. Natl Acad. Sci. USA, 2008) haverecently proposed a cooperative ligand-binding model involvingT1R1 N-terminal domain (NTD), where L-glutamate (L-glu)binds close to the hinge region, and purine nucleotides bind to anadjacent site close to the opening of the Venus flytrap domain. Tofurther understand the structural basis of umami stimulirecognition by T1R…
Characterization of ligands for human T1R3 taste receptor
2010
National audience