0000000000142254
AUTHOR
Cristina Estornut Navarro
Role of MUC4 in idiopathic pulmonary fibrosis
Background: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and irreversible form of fibrotic intersticial lung disease, characterized by uncontrolled fibroblast proliferative processes and alveolar type II epithelial dysfunction. MUC4, a multi-domain transmembrane glycoprotein, is often overexpressed in epithelial cancers, with consequences for the biological properties, involved in cellular processes related to IPF. However, the role of MUC4 in IPF has not beet studied yet. Objective: To analyze the implication of MUC4 in IPF Methods: Lung tissue from 14 healthy and 14 IPF patients was obtained. MUC4 expression was analyzed by western blot, RT-PCR and immunohistochemistry. T…
Activadores del factor de transcripción NRF2 (factor nuclear eritroide 2) en el tratamiento de la Enfermedad Pulmonar Obstructiva Crónica
La Enfermedad Pulmonar Obstructiva Crónica (EPOC) es una enfermedad respiratoria caracterizada por una disminución progresiva e irreversible de la función pulmonar producto de una respuesta inflamatoria crónica de los pulmones a partículas y gases nocivos presente en el ambiente, principalmente del humo de tabaco. El incremento prolongado del estrés oxidativo es un factor principal en la potenciación de la inflamación en EPOC. El factor nuclear eritroide 2 (NRF2) es fundamental en la citoprotección frente al estrés oxidativo al inducir la expresión de enzimas y proteínas antioxidantes y se ha visto implicado en el desarrollo de la enfermedad, por lo que puede representar una diana terapéuti…
Mechanisms of skin toxicity of paclitaxel: An in vitro preclinical assessment.
e15511 Background: Paclitaxel skin toxicity is a frequent side effect extensively evaluated in the clinical setting. However little is known about the preclinical mechanisms that lead to this toxicity. The endpoint of this study was to analyse the cutaneous mechanisms that drive paclitaxel toxicity in a preclinical model. Methods: Primary human keratinocytes were co-cultured with human dermal fibroblast in collagen gel under air-liquid interface conditions to generate a multilayered 3D epidermis. Paclitaxel was added to 3D epidermis at 0.3 µM, 3 µM and 30 µM and total RNA and protein was extracted after 24h of incubation. Markers of cell senescence (p21 and p53), anti-apoptotic mediators (…