0000000000142873

AUTHOR

Hans R. Gelderblom

showing 4 related works from this author

Chimaeric HBV core particles carrying a defined segment of Puumala hantavirus nucleocapsid protein evoke protective immunity in an animal model

1998

Abstract Hantaviruses are rodent-born agents which are pathogenic in humans causing haemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. To induce a protective immunity against a European hantavirus (Puumala) we constructed chimaeric hepatitis B virus (HBV) core particles carrying defined fragments of the Puumala virus nucleocapsid protein. After immunisation of bank voles, the natural host of Puumala virus, with core particles possessing an insertion of the N-terminal part of Puumala virus nucleocapsid protein, four of five animals were protected against subsequent virus challenge. The results show that the major protective region of the nucleocapsid protein is located …

OrthohantavirusHantavirus InfectionsRecombinant Fusion Proteinsvirusesmedicine.disease_causeVirusVirus-like particlemedicineAnimalsNucleocapsidHantavirusHepatitis B virusHantavirus pulmonary syndromeGeneral VeterinaryGeneral Immunology and MicrobiologybiologyArvicolinaePublic Health Environmental and Occupational Healthvirus diseasesViral Vaccinesbiology.organism_classificationHepatitis B Core AntigensVirologyInfectious DiseasesHepadnaviridaeMolecular MedicinePuumala virusBunyaviridaeVaccine
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Stop codon insertion restores the particle formation ability of hepatitis B virus core-hantavirus nucleocapsid protein fusions.

2003

In recent years, epitopes of various origin have been inserted into the core protein of hepatitis B virus (HBc), allowing the formation of chimeric HBc particles. Although the C-terminus of a C-terminally truncated HBc (HBcΔ) tolerates the insertion of extended foreign sequences, the insertion capacity is still a limiting factor for the construction of multivalent vaccines. Previously, we described a new system to generate HBcΔ mosaic particles based on a read-through mechanism in an <i>Escherichia coli</i> suppressor strain [J Gen Virol 1997;78:2049–2053]. Those mosaic particles allowed the insertion of a 114-amino acid (aa)-long segment of a Puumala hantavirus (PUUV) nucleocap…

Hepatitis B virusHepatitis B virus DNA polymerasevirusesRecombinant Fusion ProteinsMolecular Sequence Datamedicine.disease_causeEpitopeHepatitis B virus PRE betaMiceVirologyparasitic diseasesmedicineAnimalsNucleocapsidHantavirusHepatitis B virusMice Inbred BALB CBase SequenceChemistryHepatitis B virus coreVirionvirus diseasesNucleocapsid ProteinsVirologyMolecular biologyHepatitis B Core Antigensdigestive system diseasesStop codonNS2-3 proteaseInfectious DiseasesCodon TerminatorImmunizationIntervirology
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An amino-terminal segment of hantavirus nucleocapsid protein presented on hepatitis B virus core particles induces a strong and highly cross-reactive…

2004

AbstractPreviously, we have demonstrated that hepatitis B virus (HBV) core particles tolerate the insertion of the amino-terminal 120 amino acids (aa) of the Puumala hantavirus nucleocapsid (N) protein. Here, we demonstrate that the insertion of 120 amino-terminal aa of N proteins from highly virulent Dobrava and Hantaan hantaviruses allows the formation of chimeric core particles. These particles expose the inserted foreign protein segments, at least in part, on their surface. Analysis by electron cryomicroscopy of chimeric particles harbouring the Puumala virus (PUUV) N segment revealed 90% T = 3 and 10% T = 4 shells. A map computed from T = 3 shells shows additional density splaying out …

OrthohantavirusHepatitis B virusCryo-electron microscopyHantavirus InfectionsRecombinant Fusion ProteinsVirulenceCross Reactions030312 virologyAntibodies Viralmedicine.disease_causeCore antigenMice03 medical and health sciencesVirologymedicineAnimals030304 developmental biologyHantavirusNucleocapsid proteinchemistry.chemical_classificationHepatitis B virusMice Inbred BALB C0303 health sciencesbiologyCryoelectron MicroscopyViral VaccinesNucleocapsid ProteinsVirus-like particlesbiology.organism_classificationHepatitis B Core AntigensVirology3. Good healthAmino acidMice Inbred C57BLchemistrybiology.proteinFemalePuumala virusAntibodyHantavirus InfectionHantavirusVirology
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New chimaeric hepatitis B virus core particles carrying hantavirus (serotype Puumala) epitopes: immunogenicity and protection against virus challenge

1999

Virus-like particles generated by the heterologous expression of virus structural proteins are able to potentiate the immunogenicity of foreign epitopes presented on their surface. In recent years epitopes of various origin have been inserted into the core antigen of hepatitis B virus (HBV) allowing the formation of chimaeric HBV core particles. Chimaeric core particles carrying the 45 N-terminal amino acids of the Puumala hantavirus nucleocapsid protein induced protective immunity in bank voles, the natural host of this hantavirus. Particles applied in the absence of adjuvant are still immunogenic and partially protective in bank voles. Although a C-terminally truncated core antigen of HBV…

OrthohantavirusHantavirus InfectionsRecombinant Fusion ProteinsvirusesGenetic VectorsMolecular Sequence DataBioengineeringBiologymedicine.disease_causeRecombinant virusApplied Microbiology and BiotechnologyEpitopeVirusEpitopesVirus-like particlemedicineAnimalsHumansAmino Acid SequenceAntigens ViralHantavirusHepatitis B virusVaccines SyntheticBase SequenceArvicolinaeImmunogenicityViral VaccinesGeneral MedicineHepatitis B Core AntigensVirologyMolecular biologyHBcAgPlasmidsBiotechnologyJournal of Biotechnology
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