6533b7d1fe1ef96bd125c357
RESEARCH PRODUCT
Chimaeric HBV core particles carrying a defined segment of Puumala hantavirus nucleocapsid protein evoke protective immunity in an animal model
Gholamreza DaraiPaul SchnitzlerKatarina Brus SjölanderRainer UlrichÅKe LundkvistGalina BorisovaHelga MeiselHans R. GelderblomDiana KoletzkiDetlev H. Krügersubject
OrthohantavirusHantavirus InfectionsRecombinant Fusion Proteinsvirusesmedicine.disease_causeVirusVirus-like particlemedicineAnimalsNucleocapsidHantavirusHepatitis B virusHantavirus pulmonary syndromeGeneral VeterinaryGeneral Immunology and MicrobiologybiologyArvicolinaePublic Health Environmental and Occupational Healthvirus diseasesViral Vaccinesbiology.organism_classificationHepatitis B Core AntigensVirologyInfectious DiseasesHepadnaviridaeMolecular MedicinePuumala virusBunyaviridaedescription
Abstract Hantaviruses are rodent-born agents which are pathogenic in humans causing haemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. To induce a protective immunity against a European hantavirus (Puumala) we constructed chimaeric hepatitis B virus (HBV) core particles carrying defined fragments of the Puumala virus nucleocapsid protein. After immunisation of bank voles, the natural host of Puumala virus, with core particles possessing an insertion of the N-terminal part of Puumala virus nucleocapsid protein, four of five animals were protected against subsequent virus challenge. The results show that the major protective region of the nucleocapsid protein is located between amino acids 1 and 45 and that chimaeric HBV core-like particles are useful carries of foreign protective epitopes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-06-02 | Vaccine |