0000000000143976
AUTHOR
Gaetano Caldara
Nonsense codons suppression. An acute toxicity study of three optimized TRIDs in murine model, safety and tolerability evaluation.
Stop mutations cause 11% of the genetic diseases, due to the introduction of a premature termination codon (PTC) in the mRNA, followed by the production of a truncated protein. A promising therapeutic approach is the suppression therapy by Translational Readthrough Inducing Drugs (TRIDs), restoring the expression of the protein. Recently, three new TRIDs (NV848, NV914, NV930) have been proposed, and validated by several in vitro assays, for the rescue of the CFTR protein, involved in Cystic Fibrosis disease. In this work, an acute toxicological study for the three TRIDs was conducted in vivo on mice, according to the OECD No.420 guidelines. Animals were divided into groups and treated with …
Changes in eggshell ultrastructure of Falco naumanni and Tyto alba exposed to pesticides and polychlorinated biphenyls (PCBs)
Changes in the quality of eggs of birds exposed to environmental contaminants have been described, but few reports concern eggshell ultrastructure. In this study, infertile or addled Lesser Kestrels (Falco naumanni) and Barn owls (Tyto alba) eggs were collected from the polluted area of Gela plain (Sicily) during 2007, and compared in terms of organophosphate and organochlorine pesticides, and PCBs levels, and eggshell ultrastructure as determined by scanning electron microscopy. Pesticide and PCB residues in eggs were determined by Gas chromatography/ mass spectrometry (GC/MS) [GC Agilent 7890A/MS Agilent 5975C (Agilent technologies) using a DB-5 capillary column in the selected ion monito…
Enhanced anticancer effect of quercetin microparticles formulation obtained by spray drying
This study unravels a formulation made of food-based microparticles (MPs) able to control the release of quercetin, a natural anticancer compound, which activity is only limited by its poor aqueous solubility and consequent low bioavailability. To solve this issue, a spray-dried micro delivery system was developed using a bench mini spray dryer B290 Buchi. The resulting MPs were only manufactured with foodderived ingredients such as whey proteins and milk, avoiding the use of any other synthetic material. These microparticles were characterised with a testing campaign encompassing either the physical–chemical characterisation with SEM, DSC and DLS, or the technological and biological featur…
APHAMAX® ATTENUATES INFLAMMATORY AND OXIDATIVE STRESS IN 2, 4-DINITROBENZENE SULFONIC ACID-INDUCED COLITIS IN RAT AMELIORATING INTESTINAL FUNCTIONALITY
Accumulating evidences indicate that inflammatory and oxidative stress play an essential role in the pathogenesis and progression of inflammatory bowel disease (IBD). In IBD the excessive production of reactive oxygen species (ROS) and nitrogen metabolites contribute to tissue injury and could have also a profound impact on gut functions, including motility. We characterised the inflammatory and oxidative condition and the impact on colon motility in an experimental rat model of colitis, the 2, 4-dinitrobenzene sulfonic acid (DNBS)- induced colitis, and we evaluated if oral treatment with a nat- ural extract of Aphanizomenon flos-aquae (AFA) AphaMax®, containing concentrated quantity of AFA…