0000000000146136
AUTHOR
A. Pellicer
Profound Transcriptomic Differences Found between Sperm Samples from Sperm Donors vs. Patients Undergoing Assisted Reproduction Techniques Tends to Disappear after Swim-up Sperm Preparation Technique
Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up process in control donors and infertile males with the purpose to identify their functional significance in male fertility. Materials and methods This was a nested case-control study. Ten sperm samples were obtained from infertile patients [n=5 (two aliquots each from five samples; one before the swim-up process and one after)] and donors [n=5 (two aliquots from five samples, one before the swim-up process and one after)]. Oligonucleotide m…
Prospective, randomized study of the endometrial receptivity analysis (ERA) test in the infertility work-up to guide personalized embryo transfer versus fresh transfer or deferred embryo transfer
P–543 Inhibition of cell proliferation and extracellular matrix formation in human uterine leiomyomas by 5-aza–2’-deoxycitidine via Wnt/ β-catenin pathway
Abstract Study question Is DNA methylation reversion through DNA methyltransferases (DNMT) inhibitors, such as 5-aza–2’-deoxycitidine, a potential therapeutic option for treatment of patients with uterine leiomyomas (UL)? Summary answer 5-aza–2’-deoxycitidine reduces proliferation and extracellular matrix (ECM) formation by inhibition of Wnt/ β-catenin pathway on UL cells, suggesting DNMT inhibitors as an option to treat UL. What is known already: UL is a multifactorial disease with an unclear pathogenesis and inaccurate treatment. Aberrant DNA methylation have been found in UL compared to myometrium (MM) tissue, showing hypermethylation of tumor suppressor genes, which contributes to the d…